合成噬菌体φX 174环状病毒DNA的体外系统。
In vitro system that synthesizes circular viral DNA of bacteriophage phi X 174.
作者信息
Mukai R, Hayashi M
出版信息
J Virol. 1977 Jun;22(3):619-25. doi: 10.1128/JVI.22.3.619-625.1977.
Abstract
An extract prepared from Escherichia coli cells infected with phi chi 174 bacteriophage was capable of incorporating dTTP into phage-specific DNAs in vitro. The synthesized DNAs were associated with proteins and sedimented with S values of 20, 50, and 90 in a sucrose gradient sedimentation. DNA isolated from 20S material was open circular replicative form (RF), DNA in 50S material was replicative-form DNA with an extended single-stranded viral DNA that ranged up to one genome in length, and DNA in 90S material consisted of circular and linear single-stranded viral DNA of full genome length and single-stranded viral DNA shorter than full genome length. Pulse and pulse-chase experiments indicated that 90S material derived from 50S material.
摘要
从感染了φX174噬菌体的大肠杆菌细胞中制备的提取物能够在体外将脱氧胸苷三磷酸（dTTP）掺入噬菌体特异性DNA中。合成的DNA与蛋白质相关，并在蔗糖梯度沉降中以S值20、50和90沉降。从20S物质中分离出的DNA是开环复制形式（RF），50S物质中的DNA是具有长达一个基因组长度的延伸单链病毒DNA的复制形式DNA，90S物质中的DNA由全基因组长度的环状和线性单链病毒DNA以及短于全基因组长度的单链病毒DNA组成。脉冲和脉冲追踪实验表明，90S物质源自50S物质。
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