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合成噬菌体φX 174环状病毒DNA的体外系统。

In vitro system that synthesizes circular viral DNA of bacteriophage phi X 174.

作者信息

Mukai R, Hayashi M

出版信息

J Virol. 1977 Jun;22(3):619-25. doi: 10.1128/JVI.22.3.619-625.1977.

DOI:10.1128/JVI.22.3.619-625.1977
PMID:327087
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC515760/
Abstract

An extract prepared from Escherichia coli cells infected with phi chi 174 bacteriophage was capable of incorporating dTTP into phage-specific DNAs in vitro. The synthesized DNAs were associated with proteins and sedimented with S values of 20, 50, and 90 in a sucrose gradient sedimentation. DNA isolated from 20S material was open circular replicative form (RF), DNA in 50S material was replicative-form DNA with an extended single-stranded viral DNA that ranged up to one genome in length, and DNA in 90S material consisted of circular and linear single-stranded viral DNA of full genome length and single-stranded viral DNA shorter than full genome length. Pulse and pulse-chase experiments indicated that 90S material derived from 50S material.

摘要

从感染了φX174噬菌体的大肠杆菌细胞中制备的提取物能够在体外将脱氧胸苷三磷酸（dTTP）掺入噬菌体特异性DNA中。合成的DNA与蛋白质相关，并在蔗糖梯度沉降中以S值20、50和90沉降。从20S物质中分离出的DNA是开环复制形式（RF），50S物质中的DNA是具有长达一个基因组长度的延伸单链病毒DNA的复制形式DNA，90S物质中的DNA由全基因组长度的环状和线性单链病毒DNA以及短于全基因组长度的单链病毒DNA组成。脉冲和脉冲追踪实验表明，90S物质源自50S物质。

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In vitro system that synthesizes circular viral DNA of bacteriophage phi X 174.合成噬菌体φX 174环状病毒DNA的体外系统。
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引用本文的文献

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Role of DNA gyrase subunits in synthesis of bacteriophage phi X174 viral DNA.DNA 回旋酶亚基在噬菌体 phi X174 病毒 DNA 合成中的作用。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1532-6. doi: 10.1073/pnas.78.3.1532.
2
The J gene of bacteriophage phi X174: in vitro analysis of J protein function.噬菌体φX174的J基因：J蛋白功能的体外分析
J Virol. 1985 May;54(2):345-50. doi: 10.1128/JVI.54.2.345-350.1985.
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Proteolysis of bacteriophage phi X174 prohead protein gpB by a protease located in the Escherichia coli outer membrane.位于大肠杆菌外膜的一种蛋白酶对噬菌体phi X174前头部蛋白gpB的蛋白水解作用。
J Bacteriol. 1988 Dec;170(12):5564-71. doi: 10.1128/jb.170.12.5564-5571.1988.
4
The J gene of phi X174: isolation and characterization of a J gene mutant.噬菌体φX174的J基因：J基因突变体的分离与鉴定
Mol Gen Genet. 1988 Jan;211(1):72-7. doi: 10.1007/BF00338395.
5
DNA synthesis in Escherichia coli cells infected with gene H mutants of bacteriophage phi X174.感染噬菌体phi X174基因H突变体的大肠杆菌细胞中的DNA合成。
J Virol. 1979 Mar;29(3):973-82. doi: 10.1128/JVI.29.3.973-982.1979.
6
Synthesis of phiX174 viral DNA in vitro depends on phiX replicative form DNA.在体外合成φX174病毒DNA依赖于φX复制型DNA。
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4195-9. doi: 10.1073/pnas.74.10.4195.
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Isolation and identification of bacteriophage phi X174 prohead.噬菌体φX174原头部的分离与鉴定
Proc Natl Acad Sci U S A. 1979 Oct;76(10):4877-81. doi: 10.1073/pnas.76.10.4877.

本文引用的文献

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Phi-X-174 bacteriophage structural mutants which affect deoxyribonucleic acid synthesis.影响脱氧核糖核酸合成的Φ-X-174噬菌体结构突变体。
J Virol. 1969 Oct;4(4):400-7. doi: 10.1128/JVI.4.4.400-407.1969.
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The process of infection with Bacteriophage phi-X174. XXIX. In vivo studies on the synthesis of the single-stranded DNA of progeny phi-X174 bacteriophage.噬菌体φ-X174的感染过程。二十九。子代φ-X174噬菌体单链DNA合成的体内研究。
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Template activities of the phi X-174 replicative allomorphic deoxyribonucleic acids.φX-174复制性同素异形脱氧核糖核酸的模板活性
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DNA replication: the rolling circle model.DNA复制：滚环模型
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Stages in the replication of bacteriophage phi X174 DNA in vivo.噬菌体φX174 DNA在体内的复制阶段。
Cold Spring Harb Symp Quant Biol. 1968;33:443-7. doi: 10.1101/sqb.1968.033.01.051.
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Bacteriophage phi-X174 and related viruses.噬菌体φ-X174及相关病毒。
Prog Nucleic Acid Res Mol Biol. 1968;8:115-69.
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Gene A of X174. 1. Isolation and identification of its products.X174的基因A。1. 其产物的分离与鉴定。
Virology. 1972 Nov;50(2):381-7. doi: 10.1016/0042-6822(72)90389-3.
10
Two proteins of gene A of psiX174.噬菌体X174基因A的两种蛋白质。
Nat New Biol. 1973 Sep 5;245(140):6-8. doi: 10.1038/newbio245006a0.