Kornberg A
CRC Crit Rev Biochem. 1979 Nov;7(1):23-43. doi: 10.3109/10409237909102568.
Enzymatic mechanisms of DNA replication have been investigated using small bacteriophages as probes to illuminate the cellular systems upon which they must rely during infection. Conversion of the circular, single-stranded DNAs of phages M13, G4, and phi X174 to their duplex forms has revealed the participation of diverse ways to start a new chain and a complex DNA polymerase III holoenzyme upon which all these systems depend for chain elongation. The phi X174 system, which is the most exacting and revealing of the host chromosomal replication pattern, includes at least twenty polypeptides for making the viral DNA into a duplex and multiplying the duplex. Resolution and purification of these numerous proteins is in train and their reconstitution into a "replisome"-like structure is envisioned.
利用小型噬菌体作为探针来研究DNA复制的酶促机制，以阐明它们在感染过程中必须依赖的细胞系统。噬菌体M13、G4和φX174的环状单链DNA转化为双链形式，揭示了启动新链的多种方式以及所有这些系统在链延伸过程中所依赖的复杂DNA聚合酶III全酶的参与。φX174系统是宿主染色体复制模式中最严格且最具揭示性的，它包括至少二十种多肽，用于将病毒DNA转化为双链并复制双链。这些众多蛋白质的解析和纯化正在进行中，并且设想将它们重组为类似“复制体”的结构。
