Dresdner R D, Wong L B
ISA Trans. 1985;24(1):33-8.
Studies on the ciliary beat frequency (CBF) of frog palate epithelium are described. A high-speed video microscope system was used to record ciliary activity at 60 and 180 fields per second (fps). Temperature-controlled experiments were facilitated by a specially designed observation chamber. A total of 1150 measurements were performed on intact tissue (edge and surface observations) and single cells. The overall mean values of CBF at 60 fps (18.8 Hz) and 180 fps (19.1 Hz) were statistically indistinguishable (p less than 0.05). A temperature variation of 0.235 Hz/degree C was obtained for the range 24-27 degrees C. Comparison of results by two observers at both field rates indicated no statistical differences. The variation of CBF with time after collection was also not significant. It was determined that measurements obtained from the edge and single-cell recordings were preferable to those obtained from surface observations.
本文描述了对蛙腭上皮纤毛摆动频率（CBF）的研究。使用高速视频显微镜系统以每秒60帧和180帧（fps）的速度记录纤毛活动。通过专门设计的观察室进行温度控制实验。对完整组织（边缘和表面观察）和单个细胞总共进行了1150次测量。在60 fps（18.8 Hz）和180 fps（19.1 Hz）时CBF的总体平均值在统计学上无显著差异（p小于0.05）。在24 - 27摄氏度范围内，温度变化为0.235 Hz/摄氏度。两位观察者在两种帧率下的结果比较表明无统计学差异。采集后CBF随时间的变化也不显著。确定从边缘和单细胞记录获得的测量结果优于从表面观察获得的结果。
