School of Public Health, Chengdu Medical College, Chengdu, Sichuan, 610500, People's Republic of China.
Department of Emergency, The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, 610500, People's Republic of China.
Mol Genet Genomics. 2024 May 11;299(1):50. doi: 10.1007/s00438-024-02144-3.
Intracerebral hemorrhage (ICH) is one of the major causes of death and disability, and hypertensive ICH (HICH) is the most common type of ICH. Currently, the outcomes of HICH patients remain poor after treatment, and early prognosis prediction of HICH is important. However, there are limited effective clinical treatments and biomarkers for HICH patients. Although circRNA has been widely studied in diseases, the role of plasma exosomal circRNAs in HICH remains unknown. The present study was conducted to investigate the characteristics and function of plasma exosomal circRNAs in six HICH patients using circRNA microarray and bioinformatics analysis. The results showed that there were 499 differentially expressed exosomal circRNAs between the HICH patients and control subjects. According to GO annotation and KEGG pathway analyses, the targets regulated by differentially expressed exosomal circRNAs were tightly related to the development of HICH via nerve/neuronal growth, neuroinflammation and endothelial homeostasis. And the differentially expressed exosomal circRNAs could mainly bind to four RNA-binding proteins (EIF4A3, FMRP, AGO2 and HUR). Moreover, of differentially expressed exosomal circRNAs, hsa_circ_00054843, hsa_circ_0010493 and hsa_circ_00090516 were significantly associated with bleeding volume and Glasgow Coma Scale score of the subjects. Our findings firstly revealed that the plasma exosomal circRNAs are significantly involved in the progression of HICH, and could be potent biomarkers for HICH. This provides the basis for further research to pinpoint the best biomarkers and illustrate the mechanism of exosomal circRNAs in HICH.
脑出血（ICH）是死亡和残疾的主要原因之一，高血压性脑出血（HICH）是最常见的 ICH 类型。目前，HICH 患者的治疗后结果仍然较差，因此对 HICH 的早期预后预测很重要。然而，HICH 患者的有效临床治疗方法和生物标志物有限。尽管 circRNA 在疾病中得到了广泛研究，但血浆外泌体 circRNA 在 HICH 中的作用尚不清楚。本研究通过 circRNA 微阵列和生物信息学分析，旨在探讨 6 例 HICH 患者血浆外泌体 circRNA 的特征和功能。结果显示，HICH 患者与对照组之间存在 499 个差异表达的外泌体 circRNA。根据 GO 注释和 KEGG 通路分析，差异表达的外泌体 circRNA 的靶标与 HICH 的发展密切相关，通过神经/神经元生长、神经炎症和内皮稳态。差异表达的外泌体 circRNA 主要与四种 RNA 结合蛋白（EIF4A3、FMRP、AGO2 和 HUR）结合。此外，在差异表达的外泌体 circRNA 中，hsa_circ_00054843、hsa_circ_0010493 和 hsa_circ_00090516 与受试者的出血量和格拉斯哥昏迷量表评分显著相关。本研究结果首次表明，血浆外泌体 circRNA 显著参与 HICH 的进展，可能是 HICH 的潜在生物标志物。这为进一步研究确定最佳生物标志物和阐明外泌体 circRNA 在 HICH 中的作用机制提供了依据。
