Ekejindu G O, Shifrine M, Wilson F D, Moulton J
Trop Med Parasitol. 1985 Mar;36(1):46-52.
A semi-solid culture system was used to study the effects of trypanosome infection in two species of mice on the propagation of progenitor cells from the bone marrow and spleen. The deer mouse (Peromyscus maniculatus) survived infection with Trypanosoma (T.) equiperdum for more than 15 days. During the first 10 days there was inhibition of development of granulocyte-monocyte colonies from progenitor cells in the bone marrow. B-lymphocyte progenitors in the spleen showed increased activity, producing colonies 140-300% above normal control groups during the same period. - Conversely, all Balb/c mice infected with the trypanosomes died within 10 days with fulminating parasitemia and massive spleen enlargement. There was a general activation of progenitor cells; B-lymphocytes from the spleen and bone marrow and granulocyte-monocyte colonies from bone marrow, although this was not sustained more than 4 days after infection. - In chronically infected deer mice the pattern of response of the bone marrow and spleen progenitor cells was significantly different over successive weekly intervals. Periodicity of response in these organs was displayed by recurring waves of activation and depression of the progenitor cells. - Thus, there were significant differences in response patterns of deer mice and Balb/c mice to T. equiperdum infection which could be established by the behavior of host lymphohaematopoietic progenitor cells in culture. We therefore suggest that such in vitro cultures may be useful in assessment of the immune response to trypanosomiasis by the host and also for the study of the pathology of both chronic and acute trypanosomiasis.
采用半固体培养系统研究锥虫感染对两种小鼠骨髓和脾脏祖细胞增殖的影响。鹿鼠（白足鼠）感染马媾疫锥虫后存活超过15天。在最初的10天里，骨髓中祖细胞的粒细胞-单核细胞集落发育受到抑制。同期，脾脏中的B淋巴细胞祖细胞活性增强，产生的集落比正常对照组高出140%-300%。相反，所有感染锥虫的Balb/c小鼠在10天内死于暴发性寄生虫血症和脾脏肿大。祖细胞普遍被激活；脾脏和骨髓中的B淋巴细胞以及骨髓中的粒细胞-单核细胞集落均被激活，不过感染后这种激活状态持续不超过4天。在慢性感染的鹿鼠中，骨髓和脾脏祖细胞在连续的每周间隔内的反应模式存在显著差异。这些器官中的反应周期性表现为祖细胞激活和抑制的反复波动。因此，鹿鼠和Balb/c小鼠对马媾疫锥虫感染的反应模式存在显著差异，这可以通过宿主淋巴造血祖细胞在培养中的行为来确定。所以我们认为，这种体外培养可能有助于评估宿主对锥虫病的免疫反应，也有助于研究慢性和急性锥虫病的病理学。
