Kaushik Nikita, Noorani Md Salik, Shukla Kritika, Mirza Mohd Aamir, Dhir Sunny, Alotaibi Amenah S, Siddiqui Zahid Hameed
Plant Molecular Virology Lab, Department of Botany, School of Chemical and Life Sciences, Jamia Hamdard (A Deemed-to-Be University), New Delhi 110062, India.
Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard (A Deemed-to-Be University), New Delhi 110062, India.
ACS Omega. 2024 Apr 22;9(18):20359-20367. doi: 10.1021/acsomega.4c00877. eCollection 2024 May 7.
Malvaceae family, also known as the Mallow family, is a family of flowering plants containing and other plants of high medicinal value. This study focuses on the challenges associated with high-quality RNA extraction from and its related plants characterized by high levels of mucilage and phenolic compounds in their tissues. High mucilage and secondary metabolite content pose obstacles in obtaining high-quality RNA, negatively impacting downstream applications, such as gene expression analysis. Our research aimed to develop an efficient RNA extraction method tailored to the unique characteristics of Malvaceae family plants especially . Through the substitution of NaCl with KCl, a crucial component of the CTAB buffer, our methodology successfully addressed the challenges posed by high mucilage and phenolic compound levels. This modification led to a significant reduction in sample viscosity, which is because of the high mucilage in these plants. Our modified CTAB extraction method yielded significantly more RNA with higher purity than the conventional CTAB methods alone. The extracted RNA was largely intact, as indicated by 28/18S ratios and RIN values, yielding high-quality RNA with improved purity suggested by the 260/280 and 260/230 ratios. The proposed approach not only serves as a solution to the specific challenges encountered in but also holds promise for broader applications across different plants within the family.
锦葵科，也被称为锦葵家族，是一个开花植物家族，包含[此处原文缺失相关植物名称]以及其他具有高药用价值的植物。本研究聚焦于从[此处原文缺失相关植物名称]及其相关植物中提取高质量RNA所面临的挑战，这些植物的组织中含有高水平的黏液和酚类化合物。高黏液和次生代谢物含量在获取高质量RNA时构成障碍，对下游应用（如基因表达分析）产生负面影响。我们的研究旨在开发一种针对锦葵科植物特别是[此处原文缺失相关植物名称]独特特性的高效RNA提取方法。通过用KCl替代CTAB缓冲液的关键成分NaCl，我们的方法成功应对了高黏液和酚类化合物水平带来的挑战。这种修改导致样品黏度显著降低，这是由于这些植物中黏液含量高所致。我们改进的CTAB提取方法比单独的传统CTAB方法产生的RNA产量显著更高且纯度更高。提取的RNA在很大程度上是完整的，通过28/18S比率和RIN值表明，260/280和260/230比率表明产生了纯度更高的高质量RNA。所提出的方法不仅为[此处原文缺失相关植物名称]中遇到的特定挑战提供了解决方案，而且对该家族内不同植物的更广泛应用也具有前景。
