Department of Biotechnology, PRIST University, Vallam, Thanjavur, Tamil Nadu, India.
Indian J Pharmacol. 2012 Nov-Dec;44(6):694-8. doi: 10.4103/0253-7613.103252.
To investigate the wound-healing potency of the ethanolic extract of the flowers of Hibiscus rosa sinensis.
The wound-healing activity of H. rosa sinensis (5 and 10% w/w) on Wistar albino rats was studied using three different models viz., excision, incision and dead space wound. The parameters studied were breaking strength in incision model, granulation tissue dry weight, breaking strength and collagen content in dead space wound model, percentage of wound contraction and period of epithelization in excision wound model. The granulation tissue formed on days 4, 8, 12, and 16 (post-wound) was used to estimate total collagen, hexosamine, protein, DNA and uronic acid. Data were analyzed by Analysis of Variance (ANOVA) test. P<0.05 was considered statistically significant.
The extract increased cellular proliferation and collagen synthesis at the wound site, as evidenced by increase in DNA, total protein and total collagen content of granulation tissues. The extract-treated wounds were found to heal much faster as indicated by improved rates of epithelialization and wound contraction. The extract of H. rosa sinensis significantly (P<0.001) increased the wound-breaking strength in the incision wound model compared to controls. The extract-treated wounds were found to epithelialize faster, and the rate of wound contraction was significantly (P<0.001) increased as compared to control wounds. Wet and dry granulation tissue weights in a dead space wound model increased significantly (P<0.001). There was a significant increase in wound closure rate, tensile strength, dry granuloma weight, wet granuloma weight and decrease in epithelization period in H. rosa sinensis-treated group as compared to control and standard drug-treated groups.
The ethanolic extract of H. rosa sinensis had greater wound-healing activity than the nitrofurazone ointment.
研究木槿花乙醇提取物的创伤愈合功效。
采用三种不同模型（即切除、切开和死腔伤口），研究木槿花（5%和 10%w/w）对 Wistar 白化大鼠的创伤愈合作用。研究的参数包括切开模型中的断裂强度、肉芽组织干重、死腔伤口模型中的断裂强度和胶原含量、切除伤口模型中的伤口收缩百分比和上皮形成期。在创伤后第 4、8、12 和 16 天（创伤后）形成的肉芽组织用于估计总胶原、己糖胺、蛋白质、DNA 和糖醛酸。数据采用方差分析（ANOVA）检验进行分析。P<0.05 被认为具有统计学意义。
提取物通过增加肉芽组织中 DNA、总蛋白和总胶原含量，促进细胞增殖和胶原合成，从而在创伤部位发挥作用。提取物处理的伤口愈合速度更快，表现在上皮化和伤口收缩率的改善。与对照组相比，木槿花提取物显著（P<0.001）增加了切开伤口模型中的伤口断裂强度。与对照组相比，提取物处理的伤口更快地上皮化，伤口收缩率显著（P<0.001）增加。在死腔伤口模型中，湿和干肉芽组织重量显著增加（P<0.001）。与对照组和标准药物处理组相比，木槿花处理组的伤口闭合率、拉伸强度、干肉芽肿重量、湿肉芽肿重量和上皮形成期显著增加。
木槿花乙醇提取物的创伤愈合活性强于呋喃西林软膏。
