Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China.
Cells. 2022 Mar 30;11(7):1170. doi: 10.3390/cells11071170.
Sweet cherry, an economically important horticultural crop, has strong antioxidant activity. The fruits contain compounds potentially beneficial to human health-particularly anthocyanins, which are synthesized in cytosol and predominantly accumulated in vacuoles. Although anthocyanin levels differ among dark-red, blush, and yellow sweet cherry cultivars, the regulatory mechanism of anthocyanin transport and accumulation is not well understood in this species. In this study, we identified 53 glutathione S-transferase genes (PavGSTs) from sweet cherry and found that PavGST1 expression was well correlated with anthocyanin accumulation in cultivars with different fruit skin colors. TRV-mediated virus-induced silencing of PavGST1 decreased anthocyanin accumulation in sweet cherry fruits and downregulated the expressions of anthocyanin biosynthetic and regulatory genes. In addition, transient overexpression of PavGST1 promoted anthocyanin accumulation. Furthermore, yeast one-hybrid and dual-luciferase assays revealed that PavMYB10.1 and PavMYB75 directly bind to different MYB binding sites of the PavGST1 promoter (MBS-1 and MBS-3) to activate PavGST1 transcription. According to our results, PavGST1 plays a central role in sweet cherry fruit anthocyanin accumulation. Our findings provide novel insights into the coordinative regulatory mechanisms of PavGST1 and PavMYBs in anthocyanin accumulation in sweet cherry.
甜樱桃是一种经济价值很高的园艺作物，具有很强的抗氧化活性。其果实含有对人类健康有益的化合物，特别是花色苷，它们在细胞质中合成，并主要在液泡中积累。尽管深紫红色、粉红色和黄色甜樱桃品种的花色苷含量不同，但该物种中花色苷运输和积累的调节机制尚不清楚。本研究从甜樱桃中鉴定出 53 个谷胱甘肽 S-转移酶基因（PavGSTs），发现 PavGST1 的表达与不同果皮颜色品种的花色苷积累密切相关。TRV 介导的病毒诱导沉默 PavGST1 减少了甜樱桃果实中花色苷的积累，并下调了花色苷生物合成和调节基因的表达。此外，PavGST1 的瞬时过表达促进了花色苷的积累。此外，酵母单杂交和双荧光素酶报告基因分析表明，PavMYB10.1 和 PavMYB75 直接结合到 PavGST1 启动子的不同 MYB 结合位点（MBS-1 和 MBS-3）上，以激活 PavGST1 的转录。根据我们的结果，PavGST1 在甜樱桃果实花色苷积累中起核心作用。我们的研究结果为 PavGST1 和 PavMYBs 在甜樱桃花色苷积累中的协调调控机制提供了新的见解。
