College of Information Science and Engineering, Northeastern University, Shenyang, P. R. China.
Hebei Key Laboratory of Micro-Nano Precision Optical Sensing and Measurement Technology, Qinhuangdao, P. R. China.
Electrophoresis. 2024 Sep;45(17-18):1574-1596. doi: 10.1002/elps.202300299. Epub 2024 May 13.
Dielectrophoresis (DEP), which arises from the interaction between dielectric particles and an aqueous solution in a nonuniform electric field, contributes to the manipulation of nano and microparticles in many fields, including colloid physics, analytical chemistry, molecular biology, clinical medicine, and pharmaceutics. The measurement of the DEP force could provide a more complete solution for verifying current classical DEP theories. This review reports various imaging, fluidic, optical, and mechanical approaches for measuring the DEP forces at different amplitudes and frequencies. The integration of DEP technology into sensors enables fast response, high sensitivity, precise discrimination, and label-free detection of proteins, bacteria, colloidal particles, and cells. Therefore, this review provides an in-depth overview of DEP-based fabrication and measurements. Depending on the measurement requirements, DEP manipulation can be classified into assistance and integration approaches to improve sensor performance. To this end, an overview is dedicated to developing the concept of trapping-on-sensing, improving its structure and performance, and realizing fully DEP-assisted lab-on-a-chip systems.
介电泳（DEP）是由介电粒子与非均匀电场中的水溶液之间的相互作用引起的，它有助于在胶体物理、分析化学、分子生物学、临床医学和药剂学等多个领域中对纳米和微米粒子进行操作。DEP 力的测量可以为验证当前经典的 DEP 理论提供更完整的解决方案。本综述报告了各种用于测量不同幅度和频率下的 DEP 力的成像、流体、光学和机械方法。将 DEP 技术集成到传感器中，可以实现对蛋白质、细菌、胶体粒子和细胞的快速响应、高灵敏度、精确区分和无标记检测。因此，本综述提供了对基于 DEP 的制造和测量的深入概述。根据测量要求，DEP 操作可以分为辅助和集成方法，以提高传感器性能。为此，专门概述了开发传感辅助捕获的概念，改进其结构和性能，并实现完全 DEP 辅助的芯片上实验室系统。
