School of Agricultural Sciences, Murdoch University, Murdoch, WA, 6150, Australia.
Kanyana Wildlife Rehabilitation Centre, 120 Gilchrist Road, Lesmurdie, WA, 6076, Australia.
Syst Parasitol. 2024 May 13;101(3):40. doi: 10.1007/s11230-024-10152-5.
A novel Eimeria Schneider, 1875 species is described from an Australian pied oystercatcher Haematopus longirostris Vieillot, in Western Australia. The pied oystercatcher was admitted to the Kanyana Wildlife Rehabilitation Centre (KWRC), Perth, Western Australia in a poor body condition, abrasion to its right hock and signs of partial delamination to its lower beak. Investigation into potential medical causes resulted in a faecal sample being collected and screened for gastrointestinal parasites. Unsporulated coccidian oocysts were initially observed in the faeces and identified as Eimeria upon sporulation. The sporulated oocysts (n = 20) are ellipsoidal, 20-21 × 12-13 μm in shape and have thick bi-layered walls which are c.2/3 of the total thickness. Micropyle is present, robust and protruding, and occasionally has a rounded polar body attached to the micropyle. Within the oocyst, a residuum, in addition, two to five polar granules are present. There are four ellipsoidal sporocysts 9-11 × 5-6 μm with flattened to half-moon shaped Stieda bodies. Sub-Stieda body and para-Stieda body are absent. The sporocysts contain sporocyst residuums composed of a few spherules scattered among the sporozoites. Within the sporozoites, anterior and posterior refractile bodies are present, but the nucleus is indiscernible. To further characterise the novel Eimeria species from H. longirostris, molecular analysis was conducted at the 18S ribosomal RNA locus, using PCR amplification and cloning. Two cloned sequences from the novel Eimeria were compared with those from other Eimeria spp. with the highest genetic similarity of 97.6% and 97.2% from Clone 1 and 2, respectively with Eimeria reichenowi (AB544308) from a hooded crane (Grus monacha Temminck) in Japan. Both sequences grouped in a clade with the Eimeria spp. isolated from wetland birds, which include Eimeria paludosa (KJ767187) from a dusky moorhen (Gallinula tenebrosa Gould) in Western Australia, Eimeria reichenowi (AB544308) and Eimeria gruis (AB544336) both from hooded cranes. Based on the morphological and molecular data, this Eimeria sp. is a new species of coccidian parasite and is named Eimeria haematopusi n. sp. after its host H. longirostris.
描述了一种来自澳大利亚 pied oystercatcher Haematopus longirostris 的新型 Eimeria Schneider, 1875 种。pied oystercatcher 因身体状况不佳、右跗跖部擦伤和下喙部分分层而被送入西澳大利亚的 Kanyana 野生动物康复中心 (KWRC)。对潜在的医学病因的调查导致采集了粪便样本并进行了胃肠道寄生虫筛查。最初在粪便中观察到未孢子化的球虫卵囊，并在孢子化后鉴定为 Eimeria。孢子化的卵囊（n = 20）呈椭圆形，长 20-21μm，宽 12-13μm，具有厚的双层壁，占总厚度的 2/3。微口存在，粗壮且突出，偶尔有一个附着在微口上的圆形极体。在卵囊内，除了残留物外，还有两到五个极粒体。有四个椭圆形的孢子囊，长 9-11μm，宽 5-6μm，具有扁平至半月形的斯泰达体。无副斯泰达体和副斯泰达体。孢子囊内含有由散布在孢子虫中的几个小球体组成的孢子囊残留物。在孢子虫中，存在前、后折射体，但核不可见。为了进一步描述来自 H. longirostris 的新型 Eimeria 种，在 18S 核糖体 RNA 基因座使用 PCR 扩增和克隆进行了分子分析。从新型 Eimeria 中克隆的两个序列与来自其他 Eimeria spp. 的序列进行了比较，与来自日本 Hooded Crane (Grus monacha Temminck) 的 Eimeria reichenowi (AB544308) 的遗传相似度最高，分别为 97.6%和 97.2%。这两个序列与来自湿地鸟类的 Eimeria spp. 一起分组，包括来自西澳大利亚的 Dusky Moorhen (Gallinula tenebrosa Gould) 的 Eimeria paludosa (KJ767187)、来自 Hooded Crane 的 Eimeria reichenowi (AB544308) 和 Eimeria gruis (AB544336)。基于形态学和分子数据，该 Eimeria sp. 是一种新的球虫寄生虫种，以其宿主 H. longirostris 命名为 Eimeria haematopusi n. sp.。
