Department of Chemical Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8550, Japan.
ACS Appl Bio Mater. 2024 Jun 17;7(6):4007-4016. doi: 10.1021/acsabm.4c00359. Epub 2024 May 13.
Nanoparticles have been widely used as platforms for biomolecular sensing because of their high specific surface area and attractive properties depending on their constituents and structures. Nevertheless, it remains challenging to develop nanoparticulate sensing platforms that are easily storable without aggregation and conjugatable with various ligands in a simple manner. Herein, we demonstrate that nanoparticulate assemblies of cello-oligosaccharides with terminal azido groups are promising candidates. Azidated cello-oligosaccharides can be readily synthesized via the enzyme-catalyzed oligomerization reaction. This study characterized the assembled structures of azidated cello-oligosaccharides produced during the enzymatic synthesis and revealed that the terminal azidated cello-oligosaccharides formed rectangular nanosheet-shaped lamellar crystals. The azido groups located on the nanosheet surfaces were successfully exploited for antigen conjugation via the click chemistry. The resultant antigen-conjugated nanosheets allowed for the quantitative and specific detection of a corresponding antibody, even in 10% serum, owing to the antifouling properties of cello-oligosaccharide assemblies against proteins. It was found that the functionalized nanosheets were redispersible in water after freeze-drying. This remarkable characteristic is attributed to the well-hydrated saccharide residues on the nanosheet surfaces. Moreover, the antibody detection capability did not decline after the thermal treatment of the functionalized nanosheets in a freeze-dried state. Our findings contribute to developing convenient nanoparticulate biomolecular sensing platforms.
纳米粒子因其高比表面积和根据其组成和结构而具有的吸引力特性，已被广泛用作生物分子传感的平台。然而，开发易于储存且不会聚集的纳米颗粒传感平台，以及以简单的方式与各种配体结合的纳米颗粒传感平台仍然具有挑战性。在此，我们证明了具有末端叠氮基的纤维寡糖的纳米粒子组装体是很有前途的候选物。叠氮化纤维寡糖可以通过酶催化的聚合反应容易地合成。本研究对酶合成过程中产生的叠氮化纤维寡糖的组装结构进行了表征，并揭示了末端叠氮化纤维寡糖形成了矩形纳米片状层状晶体。纳米片表面上的叠氮基团可通过点击化学成功用于抗原偶联。由于纤维寡糖组装体对蛋白质具有抗污性质，所得的抗原偶联纳米片允许对相应的抗体进行定量和特异性检测，即使在 10%的血清中也是如此。研究发现，功能化纳米片在冷冻干燥后可再分散在水中。这种显著的特性归因于纳米片表面上高度水合的糖残基。此外，在冷冻干燥状态下对功能化纳米片进行热处理后，抗体检测能力并未下降。我们的研究结果有助于开发方便的纳米颗粒生物分子传感平台。
