Hata Yuuki, Miyazaki Hiromi, Okamoto Sayaka, Serizawa Takeshi, Nakamura Shingo
Department of Chemical Science and Engineering, School of Materials and Chemical Technology, Institute of Science Tokyo, 2-12-1-H-121 Ookayama, Meguro-ku, Tokyo 152-8550, Japan.
Division of Biomedical Engineering, National Defense Medical College Research Institute, 3-2 Namiki, Tokorozawa-shi, Saitama 359-8513, Japan.
Nano Lett. 2025 Jan 22;25(3):1177-1184. doi: 10.1021/acs.nanolett.4c05773. Epub 2025 Jan 13.
Nanostructuring surfaces is an emergent strategy to endow materials with abilities to combat pathogenic bacteria. Nevertheless, it remains challenging to create nanospike structures on the curved surfaces of polymer materials, including gauze and other microfibrous medical materials. Additionally, the effects of nanostructured surfaces on bacteria in the presence of proteins and in vivo remain largely unexplored. Herein, we demonstrated the decoration of gauze microfiber surfaces with nanospike structures via the self-assembly of cello-oligosaccharides and investigated the effects of the nanospiked gauze on bacteria in the presence of proteins. The nanospiked gauze had low bacterial adhesion properties in the absence of proteins, whereas in the presence of proteins, it promoted bacterial adhesion. Analyses suggested that the adsorbed protein layers on the nanospikes were involved in the promoted bacterial adhesion. Furthermore, the bacterial adhesion-promoting effects were exploited to remove pathogenic bacteria from burn wounds with exudate containing proteins using the nanospiked gauze.
对材料表面进行纳米结构化处理是赋予材料抗菌能力的一种新兴策略。然而，在包括纱布和其他微纤维医用材料在内的聚合物材料曲面上构建纳米刺结构仍然具有挑战性。此外，在存在蛋白质的情况下以及在体内，纳米结构化表面对细菌的影响在很大程度上仍未得到探索。在此，我们通过纤维二糖的自组装展示了在纱布微纤维表面修饰纳米刺结构，并研究了纳米刺纱布在存在蛋白质的情况下对细菌的影响。在没有蛋白质的情况下，纳米刺纱布具有较低的细菌粘附特性，而在存在蛋白质的情况下，它会促进细菌粘附。分析表明，纳米刺上吸附的蛋白质层参与了促进细菌粘附的过程。此外，利用纳米刺纱布促进细菌粘附的作用，从含有蛋白质的烧伤创面渗出物中清除病原菌。
