Griffiths A J
Environ Health Perspect. 1979 Aug;31:75-80. doi: 10.1289/ehp.793175.
Various environmental agents have been tested for their ability to produce aneuploid products of meiosis in a Neurospora cross. The cross was between two multiply marked strains designed specifically for this purpose. These parental strains were heterozygous for four auxotrophic mutations on chromosome 1. Prototrophic disomics could be selected by plating ascospores on minimal medium. Out of 48 agents tested, at least 10 increased the aneuploid frequency significantly above control levels. Some of these positive-testing agents have also been found active in other aneuploid detection systems. It is suggested that if it should become necessary to perform widespread testing of agents in the human environment for their ability to generate aneuploids at meiosis, the Neurospora system could be successfully applied for this purpose.
人们已经对多种环境因子进行了测试，以检验它们在粗糙脉孢菌杂交中产生减数分裂非整倍体产物的能力。杂交是在两个专门为此目的设计的多重标记菌株之间进行的。这些亲本菌株在1号染色体上的四个营养缺陷型突变中是杂合的。通过将子囊孢子接种在基本培养基上，可以筛选出原养型二体。在测试的48种因子中，至少有10种使非整倍体频率显著高于对照水平。其中一些呈阳性的测试因子在其他非整倍体检测系统中也被发现有活性。有人提出，如果有必要对人类环境中的因子进行广泛测试，以检测它们在减数分裂时产生非整倍体的能力，那么粗糙脉孢菌系统可成功用于此目的。
