Bond D J, McMillan L
Environ Health Perspect. 1979 Aug;31:67-74. doi: 10.1289/ehp.793167.
A system suitable for the detection of meiotic aneuploidy is described in which various different origins of the aneuploidy can be distinguished. Aneuploid meiotic products are detected as black disomic spores held in asci containing all the products of a single meiosis. Aneuploidy may result from nondisjunction or from a meiosis in which an extra replica of one of the chromosomes has been generated in some other way, e.g., extra replication. By using this system it has been shown that pFPA treatment increase aneuploidy, primarily through an effect on nondisjunction. Preliminary results with trifluralin have indicated that this compound, too, may increase aneuploidy. There is a good possibility that the system can be further developed to permit a more rapid screening using a random plating method; this will allow a more efficient two-part analysis of the effects of compounds under test.
本文描述了一种适用于检测减数分裂非整倍体的系统，该系统能够区分非整倍体的各种不同起源。非整倍体减数分裂产物被检测为保存在子囊中黑色的二体孢子，这些子囊包含单个减数分裂的所有产物。非整倍体可能源于不分离现象，或者源于减数分裂过程中某条染色体以其他方式产生了额外的复制，例如额外复制。通过使用该系统已表明，pFPA处理会增加非整倍体，主要是通过对不分离现象产生影响。氟乐灵的初步结果表明，这种化合物也可能增加非整倍体。很有可能进一步开发该系统，以便使用随机铺板法进行更快速的筛选；这将允许对受试化合物的效果进行更有效的两部分分析。
