Morpurgo G, Bellincampi D, Gualandi G, Baldinelli L, Crescenzi O S
Environ Health Perspect. 1979 Aug;31:81-95. doi: 10.1289/ehp.793181.
Two methods to detect the induction of nondisjunction with a diploid stable strain of A. nidulans are described. The first method gives only qualitative results, while the second method is quantitative and dose-effect curves can be done. Some physiological parameters affecting the induction of nondisjunction can also be studied, because either quiescent or germinating conidia can be treated with the drug under test. Some agents inducing nondisjunction were also tested for the induction of point mutation and somatic crossing-over with these comparative analysis. Two classes of agents inducing nondisjunction may be detected: the first causes all possible types of genetic damage either on quiescent or germinating conidia (a representative of this class is MMS) and acts presumably on the DNA level; the second acts only on germinating conidia and does not produce point mutation or crossing over. A representative of this class is Benomyl which interferes with spindle microtubules. A list of compounds tests is included.
描述了两种利用构巢曲霉二倍体稳定菌株检测不分离诱导的方法。第一种方法仅给出定性结果，而第二种方法是定量的，可以绘制剂量效应曲线。由于静止或萌发的分生孢子都可以用受试药物处理，因此还可以研究一些影响不分离诱导的生理参数。通过这些比较分析，还测试了一些诱导不分离的试剂对点突变和体细胞交换的诱导作用。可以检测到两类诱导不分离的试剂：第一类会对静止或萌发的分生孢子造成所有可能类型的遗传损伤（这类的一个代表是甲基磺酸甲酯），可能作用于DNA水平；第二类仅作用于萌发的分生孢子，不会产生点突变或交换。这类的一个代表是苯菌灵，它会干扰纺锤体微管。文中包含了所测试化合物的列表。
