Meyerhans A, Vartanian J P, Hultgren C, Plikat U, Karlsson A, Wang L, Eriksson S, Wain-Hobson S
Abteilung Virologie, Universität Freiburg, Germany.
J Virol. 1994 Jan;68(1):535-40. doi: 10.1128/JVI.68.1.535-540.1994.
Human immunodeficiency virus type 1 (HIV-1) replication is shown to be sensitive to the intracellular concentration of deoxynucleoside triphosphate substrates. Addition of thymidine to established cell lines resulted in a dramatic reduction of virus production. The effect could be substantially alleviated by addition of deoxycytidine, which, alone, enhanced viral titers by a factor of 2 to 3. Hydroxyurea treatment abolished HIV-1 replication in peripheral blood mononuclear cells and could be reversed by deoxyadenosine. These data show that HIV-1 replication occurs under suboptimal DNA precursor conditions.
1型人类免疫缺陷病毒（HIV-1）的复制对脱氧核苷三磷酸底物的细胞内浓度敏感。向已建立的细胞系中添加胸苷会导致病毒产生显著减少。添加脱氧胞苷可大幅减轻这种影响，单独添加脱氧胞苷可使病毒滴度提高2至3倍。羟基脲处理可消除外周血单核细胞中的HIV-1复制，脱氧腺苷可使其逆转。这些数据表明，HIV-1复制是在次优的DNA前体条件下发生的。
