Vocational School of Health Services, Inonu University, Malatya, Turkey.
Pak J Pharm Sci. 2024 Jan;37(1):25-32.
In the present study, antioxidant activity and inhibition of acetylcholinesterase (AChE) and paraoxonase (hPON 1) of Alchemilla lithophila extracts were evaluated for the first time. Besides, there is no research on the contents of phenolic compounds except for fatty acids. In this context, phenolic compounds of A. lithophila were investigated by liquid chromatography/ mass spectrometry (LC-MS/MS). The methanol extract of the A. lithophila exhibited significant inhibition on the AChE (IC value for methanol extract 0.162 ± 0.25 mg /mL, R:0.992). Besides, antioxidant activities of the A. lithophila extracts were examined using by the methods ABTS•+ and DPPH• free radical scavenging potentials, FRAP and CUPRAC metal-reducing activities. ABTS•+ and DPPH• scavenging activities were found for methanol extract at 70.67% and water extract at 75.38%, respectively. Also, FRAP and CUPRAC metal-reducing were determined for water extract 0.796 and hexane extract 1.570 as absorbance. According to LC-MS/MS analyses, the amounts of ellagic acid, catechin hydrate, gallic acid, fumaric acid, luteolin, quercetin, kaempferol, acetohydroxamic acid, caffeic acid, syringic acid, hydroxybenzoic acid and salicylic acid were determined by LC-MS/MS, respectively. As a consequence, this study will be a useful resource for determining bioactivity and phenolic compound profile for natural medicine research.
本研究首次评价了岩风 Alchemilla lithophila 提取物的抗氧化活性和乙酰胆碱酯酶（AChE）及对氧磷酶（hPON1）抑制活性。此外，除了脂肪酸之外，目前尚无关于酚类化合物含量的研究。在这方面，通过液相色谱/质谱联用（LC-MS/MS）对 A. lithophila 的酚类化合物进行了研究。A. lithophila 的甲醇提取物对 AChE 表现出显著的抑制作用（甲醇提取物的 IC 值为 0.162 ± 0.25 mg/mL，R：0.992）。此外，还通过 ABTS•+和 DPPH•自由基清除能力、FRAP 和 CUPRAC 金属还原活性测定法来研究 A. lithophila 提取物的抗氧化活性。甲醇提取物对 ABTS•+的清除活性为 70.67%，水提取物对 DPPH•的清除活性为 75.38%。此外，水提取物的 FRAP 和 CUPRAC 金属还原能力分别为 0.796 和 1.570（吸光度）。根据 LC-MS/MS 分析，通过 LC-MS/MS 分别测定了鞣花酸、儿茶素水合物、没食子酸、富马酸、木樨草素、槲皮素、山奈酚、乙酰氧肟酸、咖啡酸、丁香酸、羟基苯甲酸和水杨酸的含量。因此，本研究将为天然药物研究中确定生物活性和酚类化合物图谱提供有用的资源。
