Kapsenberg M L, Stiekema F, Leene W
Immunology. 1985 Jun;55(2):301-10.
Dendritic cells have been isolated from rabbit lymph nodes. Morphologically and phenotypically, they resemble dendritic cells from the mouse and rat. A comparison was made of the accessory cell function of dendritic cells and peritoneal macrophages in T-cell proliferation induced by phytohaemagglutinin (PHA) or Con A, or by a simultaneous treatment with the enzymes neuraminidase and galactose oxidase (NaGo). Dendritic cells seemed to be more effective than macrophages as accessory cells in these assays. However, macrophages suppress lymphocyte proliferation through the release of oxidating agents and production of prostaglandins. Elimination of this suppressive effect of the macrophages by addition of a combination of 2-mercaptoethanol (2-ME) and indomethacin in PHA-induced cell proliferation resulted in a much higher support by macrophages, giving results that were comparable to those obtained with dendritic cells, but in NaGo-induced proliferation, macrophages were still not as effective as dendritic cells in the presence of the drugs. Experiments in diffusion culture vessels and with interleukin-1-containing macrophage supernatants showed that support by accessory cells can be mediated by soluble factors in PHA-induced proliferation. In contrast, in NaGo-induced proliferation, lymphocytes and accessory cells have to interact directly.
已从兔淋巴结中分离出树突状细胞。在形态和表型上，它们类似于来自小鼠和大鼠的树突状细胞。对树突状细胞和腹腔巨噬细胞在由植物血凝素（PHA）或刀豆蛋白A（Con A）诱导的T细胞增殖中，或在同时用神经氨酸酶和半乳糖氧化酶（NaGo）处理诱导的T细胞增殖中的辅助细胞功能进行了比较。在这些试验中，树突状细胞作为辅助细胞似乎比巨噬细胞更有效。然而，巨噬细胞通过释放氧化剂和产生前列腺素抑制淋巴细胞增殖。在PHA诱导的细胞增殖中，通过添加2-巯基乙醇（2-ME）和吲哚美辛的组合消除巨噬细胞的这种抑制作用，导致巨噬细胞的支持作用大大增强，其结果与用树突状细胞获得的结果相当，但在NaGo诱导的增殖中，在有药物存在的情况下，巨噬细胞仍然不如树突状细胞有效。在扩散培养容器中以及用含白细胞介素-1的巨噬细胞上清液进行的实验表明，在PHA诱导的增殖中，辅助细胞的支持作用可由可溶性因子介导。相反，在NaGo诱导的增殖中，淋巴细胞和辅助细胞必须直接相互作用。
