de Weck A L, Stadler B M, Knutti-Müller J, Ruff P, Hofstetter H, Lüdin C, Heusser C
Int Arch Allergy Appl Immunol. 1985;77(1-2):38-44. doi: 10.1159/000233750.
Several pitfalls may affect studies on human IgE synthesis in vitro. In this paper, the requirement for stringent specificity of the anti-IgE antibodies used and for assessment not only of IgE detectable in culture supernatants but also as cell-associated IgE is emphasized. The use of cycloheximide-treated cultures as controls also leaves wishes open. Activated, human T cells and T cell hybridomas produce IgE-binding factors, which may be detected by a sensitive in vitro test and which may apparently also become the endeavour of synthesis by molecular biological techniques. Although the evidence available in rodents for the role of IgE-binding factors in modulating IgE synthesis has not yet been fully reproduced by us in man, the fact that classical IgE-enhancing procedures in rodents (e.g. radiotherapy, T cell suppression) also affect IgE production in man leads to believe that similar immunoregulation mechanisms apply to various mammalian species studied so far.
有几个陷阱可能会影响体外人类IgE合成的研究。本文强调了所使用的抗IgE抗体严格特异性的要求，以及不仅要评估培养上清液中可检测到的IgE，还要评估细胞相关IgE的必要性。使用环己酰亚胺处理的培养物作为对照也存在问题。活化的人类T细胞和T细胞杂交瘤会产生IgE结合因子，这些因子可以通过灵敏的体外试验检测到，并且显然也可能成为分子生物学技术合成的目标。尽管我们尚未在人类中完全重现啮齿动物中关于IgE结合因子在调节IgE合成中作用的现有证据，但啮齿动物中经典的IgE增强程序（如放疗、T细胞抑制）也会影响人类IgE产生这一事实，使人们相信类似的免疫调节机制适用于迄今为止研究的各种哺乳动物物种。
