Faculty of Veterinary Medicine, Institute of Virology, Leipzig University, Leipzig, Germany.
Faculty of Veterinary Medicine, Institute of Veterinary Pathology, Leipzig University, Leipzig, Germany.
Methods Mol Biol. 2024;2808:153-165. doi: 10.1007/978-1-0716-3870-5_12.
Domestic cats are the natural host of feline morbilliviruses (FeMV). Although other species can also be infected (such as dogs and opossums), no laboratory animal infection model is established so far. In vitro models for studying the molecular pathogenesis are therefore needed. For this purpose, propagation and titration of FeMV are key techniques. Unlike other morbilliviruses, such as canine distemper virus (CDV) or measles virus (MV), FeMV is a slow growing virus in cell culture and is difficult to titrate using classical plaque techniques. Here we describe methods for the efficient isolation of FeMV from natural sources (e.g., urine), the propagation of viral stocks, and their titration. In addition, we establish the generation of a three-dimensional infection model mimicking the feline tubular epithelium.
家猫是猫麻疹病毒（FeMV）的天然宿主。虽然其他物种也可能被感染（如狗和负鼠），但目前尚未建立实验室动物感染模型。因此，需要建立体外模型来研究分子发病机制。为此，FeMV 的繁殖和滴定是关键技术。与其他麻疹病毒（如犬瘟热病毒（CDV）或麻疹病毒（MV））不同，FeMV 在细胞培养中生长缓慢，并且难以使用经典的噬斑技术进行滴定。在这里，我们描述了从天然来源（例如尿液）中分离 FeMV、繁殖病毒库存及其滴定的有效方法。此外，我们建立了一种模拟猫管状上皮的三维感染模型。
