Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA.
Methods Mol Biol. 2024;2808:247-264. doi: 10.1007/978-1-0716-3870-5_18.
Measles IgG avidity assays determine the overall strength of molecular binding between measles-specific IgG antibodies and measles virus antigens. Avidity results can distinguish recent from distant measles virus infections. Individuals who are immunologically naïve to measles virus develop low-avidity antibodies upon measles virus infection or first-time vaccination. Within 4-6 months, antibodies mature to high avidity. Measles avidity assays are most useful in the context of measles elimination. In such settings, avidity and epidemiological and clinical information are used to classify measles breakthrough infections for control and surveillance purposes and to assist in case confirmation when other laboratory results are inconclusive or nonexistent. We present a highly accurate end-titer measles avidity assay that delivers results based on IgG quality (avidity) that are independent of IgG concentration.
麻疹 IgG 亲和性测定法可确定麻疹特异性 IgG 抗体与麻疹病毒抗原之间的整体分子结合强度。亲和性结果可区分近期和远期麻疹病毒感染。麻疹病毒初次感染或初次接种疫苗的免疫无应答者会产生低亲和力抗体。4-6 个月内，抗体成熟为高亲和力。在消除麻疹的背景下，麻疹亲和性测定最有用。在这种情况下，亲和性以及流行病学和临床信息可用于对麻疹突破性感染进行分类，以进行控制和监测，并在其他实验室结果不确定或不存在时协助病例确认。我们提出了一种高度准确的终点滴度麻疹亲和性测定法，该方法基于 IgG 质量（亲和力）而不是 IgG 浓度提供结果。
