Jia Bao-Zhu, Chen Feng-Yan, Yang Xing-Xing, Hongsibsong Surat, Wang Xing-Xing, Xu Zhen-Lin, Luo Lin
College of Biology and Food Engineering, Guangdong University of Education, Guangzhou 510303, China; School of Health Sciences Research, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand.
College of Biology and Food Engineering, Guangdong University of Education, Guangzhou 510303, China; Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Food Chem. 2024 Sep 15;452:139580. doi: 10.1016/j.foodchem.2024.139580. Epub 2024 May 8.
The absence of high-affinity antibodies has hindered the development of satisfactory immunoassays for dichlorvos (DDVP) and trichlorfon (TCP), two highly toxic organophosphorus pesticides. Herein, the de novo synthesis of a novel anti-DDVP hapten was introduced. Subsequently, a specific anti-DDVP monoclonal antibody (Mab) was produced with satisfying affinity to DDVP (IC: 12.4 ng mL). This Mab was highly specific to DDVP, and TCP could readily convert into DDVP under mild alkaline conditions. Leveraging this insight, an indirect competitive ELISA was successfully developed for simultaneous detection of DDVP and TCP. The limit of detection in rice, cabbage and apple for DDVP /TCP was found to be 12.1/14.6 μg kg, 7.3/8.8 μg kg and 6.9/8.3 μg kg, respectively. This study not only provides an effective strategy for producing a high-quality anti-DDVP Mab but also affords a reliable and cost-effective tool suitable for high-throughput detection of DDVP and TCP in food samples.
缺乏高亲和力抗体阻碍了针对敌敌畏（DDVP）和敌百虫（TCP）这两种剧毒有机磷农药开发出令人满意的免疫分析方法。在此，引入了一种新型抗敌敌畏半抗原的从头合成方法。随后，制备了一种对敌敌畏具有令人满意亲和力（IC：12.4 ng/mL）的特异性抗敌敌畏单克隆抗体（Mab）。该单克隆抗体对敌敌畏具有高度特异性，且敌百虫在温和碱性条件下可轻易转化为敌敌畏。基于这一认识，成功开发了一种间接竞争ELISA法用于同时检测敌敌畏和敌百虫。在大米、卷心菜和苹果中，敌敌畏/敌百虫的检测限分别为12.1/14.6 μg/kg、7.3/8.8 μg/kg和6.9/8.3 μg/kg。本研究不仅为制备高质量抗敌敌畏单克隆抗体提供了有效策略，还提供了一种可靠且经济高效的工具，适用于食品样品中敌敌畏和敌百虫的高通量检测。
