Department of Pharmaceutical Engineering, School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, 510006, People's Republic of China.
Mikrochim Acta. 2024 May 14;191(6):330. doi: 10.1007/s00604-024-06304-5.
In view of a large number of people infected with Helicobacter pylori (H. pylori) with great harm followed, there is an urgent need to develop a non-invasive, easy-to-operate, and rapid detection method, and to identify effective sterilization strategies. In this study, highly specific nanoprobes with nanozyme activity, Ag@Pt nanoparticles (NPs) with the antibody, were utilized as a novel lateral flow immunoassay (LFIA). The optical label (Ag@Pt NPs) was enhanced by the introduction of the chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB) and compared with a gold nanoparticles (Au NPs) optical label. Under the optimal condition, Ag@Pt-LFIA and TMB-enhanced Ag@Pt-LFIA for H. pylori were successfully established, two of which were over twofold and 100-fold more sensitive than conventional visual Au NP-based LFIA, respectively. Furthermore, Ag@Pt NPs with the antibody irradiated with NIR laser (808 nm) at a power intensity of 550 mW/cm for 5 min exhibited a remarkable antibacterial effect. The nanoprobes could close to bacteria through effective interactions between antibodies and bacteria, thereby benefiting photothermal sterilization. Overall, Ag@Pt NPs provide promising applications in pathogen detection and therapeutic applications.
鉴于大量感染幽门螺杆菌 (H. pylori) 的人受到极大的伤害，因此迫切需要开发一种非侵入性、易于操作和快速的检测方法，并确定有效的杀菌策略。在这项研究中，利用具有纳米酶活性的高度特异性纳米探针——抗体修饰的 Ag@Pt 纳米颗粒 (NPs) 作为一种新型的侧向流动免疫分析 (LFIA)。通过引入显色底物 3,3',5,5'-四甲基联苯胺 (TMB)，对光学标记物 (Ag@Pt NPs) 进行了增强，并与金纳米颗粒 (Au NPs) 光学标记物进行了比较。在最佳条件下，成功建立了用于检测 H. pylori 的 Ag@Pt-LFIA 和 TMB 增强型 Ag@Pt-LFIA，其中两种方法的灵敏度分别比传统的基于视觉 Au NP 的 LFIA 高两倍和 100 倍。此外，用 808nm 近红外激光 (550mW/cm2) 辐照抗体修饰的 Ag@Pt NPs 5 分钟后表现出显著的抗菌效果。纳米探针可以通过抗体与细菌的有效相互作用接近细菌，从而有利于光热杀菌。总体而言，Ag@Pt NPs 在病原体检测和治疗应用方面具有广阔的应用前景。
