Wang Tiantian, Li Xiangguang, Chen Lili, Zhang Youhuan, Zheng Yujun, Yu Linjin, Ye Zhiyu, Wang Huaqian, Cui Xiping, Zhao Suqing
Department of Pharmaceutical Engineering, School of Biomedical and Pharmace-utical Sciences, Guangdong University of Technology, Guangzhou 510006, People's Republic of China.
Analyst. 2021 Jan 4;146(1):338-347. doi: 10.1039/d0an01533d.
As the infection by Helicobacter pylori (H. pylori, HP) remains for a lifetime and may induce diseases such as gastric cancer, it is vital to detect and diagnose it. A new non-invasive indirect enzyme-linked immunosorbent assay (iELISA) method based on nano-flowers (NFs) is very advantageous for the sensitive detection of HP. Furthermore, the established iELISA method based on the organic-inorganic bifunctional hybrid nano-flowers including rabbit polyclonal antibody of HP labeled with peroxidase from horseradish (R-HP-Ab-HRP@Cu2+ NFs) showed linearity with HP at a concentration of 0-105 CFU mL-1 (R2 = 0.9997). Moreover, the limit of detection (LOD) reached 50 CFU mL-1, and not only was the detection sensitivity 20 times higher than that based on rabbit polyclonal antibody of HP labeled with peroxidase from horseradish (R-HP-Ab-HRP) but also the stability of R-HP-Ab-HRP in NFs was improved. In addition, the OD450 nm value was still linearly related to the concentration of HP at a range of 0-105 CFU mL-1 (R2 = 0.9952) with a LOD of 50 CFU mL-1 in an artificial saliva system. This study provided a sensitive, low-cost and convenient method for the non-invasive detection of HP.
由于幽门螺杆菌（H. pylori，HP）感染会终生存在且可能诱发胃癌等疾病，因此对其进行检测和诊断至关重要。一种基于纳米花（NFs）的新型非侵入性间接酶联免疫吸附测定（iELISA）方法对于HP的灵敏检测非常有利。此外，所建立的基于有机-无机双功能杂化纳米花的iELISA方法，即包含用辣根过氧化物酶标记的HP兔多克隆抗体（R-HP-Ab-HRP@Cu2+ NFs），在HP浓度为0-105 CFU mL-1时显示出线性关系（R2 = 0.9997）。而且，检测限（LOD）达到50 CFU mL-1，不仅检测灵敏度比基于用辣根过氧化物酶标记的HP兔多克隆抗体（R-HP-Ab-HRP）的方法高20倍，而且R-HP-Ab-HRP在纳米花中的稳定性也得到了提高。此外，在人工唾液系统中，OD450 nm值在0-105 CFU mL-1范围内仍与HP浓度呈线性相关（R2 = 0.9952），检测限为50 CFU mL-1。本研究为HP的非侵入性检测提供了一种灵敏、低成本且便捷的方法。
