Kawai T, Kato A, Higashi H, Kato S, Naiki M
Department of Biochemistry, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Cancer Res. 1991 Feb 15;51(4):1242-6.
N-Glycolylneuraminic acid (NeuGc) is distributed in most animals except humans and chickens. However, human and chicken cancerous tissues often synthesize this heterophilic sialic acid as a tumor-associated Hanganutziu-Deicher antigen [M. Naiki and H. Higashi, Adv. Exp. Med. Biol., 152: 445-456, 1982; H. Higashi et al., Cancer Res., 45: 3796-3802, 1985]. In this paper, NeuGc in human cancerous tissues and chicken Marek's disease lymphoma cell lines was determined quantitatively with gas chromatography-mass spectrometry analysis using mass fragmentography. The detectable limit of NeuGc was 40 pg (0.12 pmol) in each injection using 5 ng of trideuteriomethyl ester trideuteriomethyl glycoside of the sialic acid as an internal standard sample when a pair of ions at m/e 386 and 389 was chosen for ion monitoring. NeuGc was detected in ganglioside-rich fractions of various human cancerous tissues from 5 of 8 patients examined but was not detected in glycosphingolipids of normal human tissues. The contents of NeuGc in these cancerous tissues ranged from 0.02 to 0.5% of the total sialic acid content. NeuGc was also detected in freeze-dried samples of 5 different cell lines from chicken Marek's disease lymphomas but was not detected in a cell line from chicken lymphoid leukosis lymphoma and normal chicken skeletal muscle tissue. The contents of NeuGc in the positive cell lines ranged from 0.03 to 0.11% of the total sialic acid content. These results indicate that NeuGc can be synthesized in both humans and chickens in some cancers.
N-羟乙酰神经氨酸（NeuGc）分布于除人类和鸡以外的大多数动物体内。然而，人类和鸡的癌组织常常合成这种嗜异性唾液酸，作为肿瘤相关的汉加努齐乌-戴歇尔抗原[M. 内木和H. 东，《实验医学与生物学进展》，152: 445 - 456，1982；H. 东等人，《癌症研究》，45: 3796 - 3802，1985]。在本文中，采用质量碎片色谱法通过气相色谱 - 质谱分析对人类癌组织和鸡马立克氏病淋巴瘤细胞系中的NeuGc进行了定量测定。当选择m/e 386和389处的一对离子进行离子监测时，以唾液酸的三氘代甲酯三氘代甲基糖苷作为内标样品，每次进样时NeuGc的检测限为40 pg（0.12 pmol）。在所检测的8例患者中的5例的各种人类癌组织富含神经节苷脂的组分中检测到了NeuGc，但在正常人体组织的糖鞘脂中未检测到。这些癌组织中NeuGc的含量占总唾液酸含量的0.02%至0.5%。在鸡马立克氏病淋巴瘤的5种不同细胞系的冻干样品中也检测到了NeuGc，但在鸡淋巴细胞性白血病淋巴瘤细胞系和正常鸡骨骼肌组织中未检测到。阳性细胞系中NeuGc的含量占总唾液酸含量的0.03%至0.11%。这些结果表明，在某些癌症中，人类和鸡都能合成NeuGc。
