一种用于检测六味木香胶囊中天仙子苷、鞣花酸、胡椒碱、木香烃内酯和去氢木香内酯的高效液相色谱法。

A validated high-performance liquid chromatography method for detecting geniposide, ellagic acid, piperine, costunolide and dehydrocostus lactone in Liuwei Muxiang capsules.

机构信息

Jiangshan Hospital of Traditional Chinese Medicine.

Fuan Pharmaceutical Group Ningbo Team Pharmaceutical Co., Ltd, Ningbo.

出版信息

Pak J Pharm Sci. 2024 Jan;37(1(Special)):205-213.

Abstract

In this study, a sensitive high-performance liquid chromatography detector was established and validated for the simultaneous determination of geniposide, ellagic acid, piperine, costunolide and dehydrocostuslactone in Liuwei Muxiang Capsules. The analysis was achieved on CHANIN 100-5-C18-H column (5μm, 250 mm×4.6 mm) with the temperature of 30C. Gradient elution was applied using 0.1% phosphoric acid solution-methanol-acetonitrile (50:50) as mobile phase at the flow rate of 1.0 mL/min. The determination was performed at the wavelength of 225 nm (detecting geniposide), 254 nm (detecting ellagic acid), 343 nm (detecting piperine) and 225 nm (detecting costunolide and dehydrocostuslactone) along with the sample volume of 10μL. The linear ranges of geniposide, ellagic acid, piperine, costunolide and dehydrocostuslactone demonstrated good linear relationships within their respective determination ranges. The average recoveries were 100.04%, 99.86%, 99.79%, 100.17% and 100.41%, respectively. RSD% was 1.3%, 1.2%, 1.2%, 1.2%, 1.5%, respectively. The developed method was proved to be simple, accurate and sensitive, which can provide a quantitative analysis method for the content determination of geniposide, ellagic acid, piperine, costunolide and dehydrocostuslactone in Liuwei Muxiang capsules.

摘要

本研究建立并验证了一种灵敏的高效液相色谱检测法，用于同时测定六味木香胶囊中的栀子苷、鞣花酸、胡椒碱、木香烃内酯和去氢木香内酯。分析采用 CHANIN 100-5-C18-H 柱（5μm，250mm×4.6mm），温度为 30°C。梯度洗脱采用 0.1%磷酸溶液-甲醇-乙腈（50:50）作为流动相，流速为 1.0mL/min。栀子苷在 225nm 波长、鞣花酸在 254nm 波长、胡椒碱在 343nm 波长、木香烃内酯和去氢木香内酯在 225nm 波长下检测，进样量为 10μL。栀子苷、鞣花酸、胡椒碱、木香烃内酯和去氢木香内酯的线性范围在各自的测定范围内均呈现良好的线性关系。栀子苷、鞣花酸、胡椒碱、木香烃内酯和去氢木香内酯的平均回收率分别为 100.04%、99.86%、99.79%、100.17%和 100.41%，RSD%分别为 1.3%、1.2%、1.2%、1.2%和 1.5%。所建立的方法简单、准确、灵敏，可为六味木香胶囊中栀子苷、鞣花酸、胡椒碱、木香烃内酯和去氢木香内酯的含量测定提供定量分析方法。