[高效液相色谱法测定不同藏区藏药当佐中的四种成分]

[HPLC determination of four components in Tibetan medicine Dangzuo of different Tibetan regions].

作者信息

Li Cen, Xiao Yuancan, Yang Hongxia, Du Yuzhi, Wei Lixin

机构信息

Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810008, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2011 Apr;36(8):1046-9.

PMID:21809583

Abstract

OBJECTIVE

To develop an HPLC method for determination of gallic acid, hydroxysafflor yellow A, cinnamic aldehyde and piperine in Tibetan medicine Dangzuo, and to compare the content of four active components in Dangzuo of different Tibetan regions.

METHOD

The separation was carried out on a Waters XTerra RP-C18 column ( 4.6 mm x 250 mm, 5 microm). The mobile phases were methanol and water, all contained 0.1% glacial acetic acid, for gradient elution. The gradient program was as follows: 0-22.5 min, methanol was changed from 5% to 50%; 22.5-40 min, changed to 80% 80:20. The flow rate was 1.0 mL x min(-1). The detection wavelength was 270 nm. The reference wavelength was 500 nm.

RESULT

The linear ranges of gallic acid, hydroxysafflor yellow A, cinnamic aldehyde and piperine were 0.040-0.640 microg (r = 0.999 8), 0.090-1.440 microg (r = 0.999 9), 0.031-0.500 microg (r = 0.999 9 ) and 0.092-41.477 microg (r = 0.998 9), respectively. The average recoveries (n = 6) were 97.42% (RSD 1.9%), 97.55% (RSD 2.9%), 98.69% (RSD 0.96%) and 96.72% (RSD 4.0%), respectively. The content ranges of gallic acid, hydroxysafflor yellow A, cinnamic aldehyde and piperine in Dangzuo samples of different Tibetan regions were 0.11341.69 mg x g(-1), 0.889-1.51 mg x g(-1), 0.000-40.606 mg x g(-1) and 1.96-2.73 mg x g(-1), respectively.

CONCLUSION

The method is a simple and effective for quality control of Tibetan medicine Dangzuo.

摘要

目的

建立高效液相色谱法测定藏药当佐中没食子酸、羟基红花黄色素A、桂皮醛和胡椒碱的含量，并比较不同藏区当佐中4种活性成分的含量。

方法

采用Waters XTerra RP-C18色谱柱（4.6 mm×250 mm，5μm）分离。流动相为甲醇和水，均含0.1%冰醋酸，进行梯度洗脱。梯度程序如下：0～22.5 min，甲醇由5%变为50%；22.5～40 min，变为80%（甲醇∶水=80∶20）。流速为1.0 mL·min-1。检测波长为270 nm，参比波长为500 nm。

结果

没食子酸、羟基红花黄色素A、桂皮醛和胡椒碱的线性范围分别为0.040～0.640μg（r = 0.999 8）、0.090～1.440μg（r = 0.999 9）、0.031～0.500μg（r = 0.999 9）和0.092～41.477μg（r = 0.998 9）。平均回收率（n = 6）分别为97.42%（RSD 1.9%）、97.55%（RSD 2.9%）、98.69%（RSD 0.96%）和96.72%（RSD 4.0%）。不同藏区当佐样品中没食子酸、羟基红花黄色素A、桂皮醛和胡椒碱的含量范围分别为0.113～41.69 mg·g-1、0.889～1.51 mg·g-1、0.000～40.606 mg·g-1和1.96～2.73 mg·g-1。