Kashiwa H, Hyodo S, Kishi T, Karakawa T, Kittaka E, Suzawa T, Sakano T, Usui T
Clin Exp Immunol. 1985 Jun;60(3):509-17.
We demonstrated that a primary exposure to the lymphocytosis promoting factor (LPF) of Bordetella pertussis-induced T cell colony formation. Colony formation was observed when mononuclear cells (MNC) were cultured at concentrations of more than 1 X 10(6)/ml, and reached a peak on day 8. However, the number of colonies generated with LPF was about one-third induced with phytohaemagglutinin (PHA). Removal of monocytes from MNC or T cells resulted in the failure of colony formation, but colony growth could be restored by the addition of monocytes or B enriched cells, indicating that they were required for the optimal colony growth induced by LPF. In the absence of accessory cells, optimal colony growth from monocyte depleted T cells could be obtained when an appropriate concentration of phorbol myristate acetate (PMA) or interleukin-2 (IL-2) was added in the cultures with LPF. PMA did not enhance LPF-induced colony formation in the cultures containing a sufficient amount of exogeneous IL-2. These findings suggest that IL-2 is essential to LPF-induced colony formation. Surface marker analysis showed that most of LPF-induced colony cells were T cells. The percentages of T4+ and T gamma cells of LPF-induced colony cells were more, and T8+ cells less, than those of PHA-induced colony cells. Ia1, T9 and Tac antigens were detected on many colony cells induced by LPF or PHA. These results indicate that the phenotype of LPF-induced colony cells differs from those of PHA, but the sequential antigen expression on lymphocytes triggered by IL-2 might be similar in both LPF- and PHA-induced colony formation.
我们证明，初次接触百日咳博德特氏菌的淋巴细胞增多促进因子（LPF）可诱导T细胞集落形成。当单核细胞（MNC）以高于1×10⁶/ml的浓度培养时可观察到集落形成，并在第8天达到峰值。然而，LPF诱导产生的集落数量约为植物血凝素（PHA）诱导数量的三分之一。从MNC或T细胞中去除单核细胞会导致集落形成失败，但通过添加单核细胞或富含B细胞可恢复集落生长，这表明它们是LPF诱导最佳集落生长所必需的。在没有辅助细胞的情况下，当在含有LPF的培养物中添加适当浓度的佛波醇肉豆蔻酸酯乙酸酯（PMA）或白细胞介素-2（IL-2）时，可从单核细胞耗竭的T细胞中获得最佳集落生长。在含有足够量外源性IL-2的培养物中，PMA不会增强LPF诱导的集落形成。这些发现表明IL-2对LPF诱导的集落形成至关重要。表面标志物分析显示，大多数LPF诱导的集落细胞是T细胞。LPF诱导的集落细胞中T4⁺和Tγ细胞的百分比高于PHA诱导的集落细胞，而T8⁺细胞的百分比则较低。在许多由LPF或PHA诱导的集落细胞上检测到Ia1、T9和Tac抗原。这些结果表明，LPF诱导的集落细胞的表型与PHA诱导的不同，但在LPF和PHA诱导的集落形成中，由IL-2触发的淋巴细胞上的顺序抗原表达可能相似。
