Suzawa T, Hyodo S, Kishi T, Kashiwa H, Kittaka E, Sakano T, Usui T
Immunology. 1983 Dec;50(4):575-80.
Colony growth of human lymphocytes by phorbol myristate acetate (PMA) was studied. PMA was able to induce lymphocyte colony growth in methylcellulose semi-solid cultures in the absence of lectin mitogens, phytohaemagglutinin or concanavalin A. PMA-induced colonies were found in cultures of mononuclear cells, monocyte-depleted mononuclear cells and the T-enriched cell fraction, whereas no colonies were obtained from the non-T cell fraction. At least one million mononuclear cells were required to form colonies by PMA. The colony cells were mainly T cells as judged from sheep red blood cell rosette formation. Surface immunoglobulin positive cells and peroxidase positive cells were not detected in colony cells. Non-specific esterase positive cells were only found to be less than 1% of colony cells. T cells formed more colonies than did mononuclear cells, presumably because of a concentration of colony forming cells and/or co-operating cells. Colony formation by PMA was induced from monocyte-depleted mononuclear cells and monocyte-depleted T cells, suggesting independence of monocytes. When mononuclear cells were precultured at 37 degrees for 5 min or 30 min with PMA and then cultured in the semi-solid medium without PMA, only a small number of colonies grew. Further studies of PMA-induced T-cell colonies will provide information the identification and characterization of immunological states in various immunological diseases.
研究了佛波酯（PMA）对人淋巴细胞集落生长的影响。在无凝集素促有丝分裂原、植物血凝素或刀豆蛋白A的情况下，PMA能够在甲基纤维素半固体培养物中诱导淋巴细胞集落生长。在单核细胞、去除单核细胞的单核细胞和富含T细胞的细胞组分培养物中发现了PMA诱导的集落，而从非T细胞组分中未获得集落。PMA形成集落至少需要100万个单核细胞。从绵羊红细胞玫瑰花结形成判断，集落细胞主要是T细胞。在集落细胞中未检测到表面免疫球蛋白阳性细胞和过氧化物酶阳性细胞。非特异性酯酶阳性细胞仅占集落细胞的不到1%。T细胞比单核细胞形成更多的集落，可能是因为集落形成细胞和/或协同细胞的浓度较高。PMA诱导的集落形成是从去除单核细胞的单核细胞和去除单核细胞的T细胞中诱导的，表明与单核细胞无关。当单核细胞在37℃下用PMA预培养5分钟或30分钟，然后在无PMA的半固体培养基中培养时，只有少数集落生长。对PMA诱导的T细胞集落的进一步研究将为各种免疫疾病中免疫状态的鉴定和表征提供信息。
