Hou Dongjie, Lu Ling, Tao Ran
Medical College of Soochow University, Suzhou, China.
Center for Plastic & Reconstructive Surgery, Department of Hand & Reconstructive Surgery, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, China.
Cartilage. 2024 May 15:19476035241250198. doi: 10.1177/19476035241250198.
Knee osteoarthritis (KOA) is a complex degenerative joint disease and a major cause of joint dysfunction. This study aimed to explore the function of hsa_circ_0007482 on inflammation, proliferation, differentiation, and apoptosis in KOA.
Real-time quantitative polymerase chain reaction (PCR) was performed to detect the expression of circ_0007482, inflammatory factors, and differentiation-related molecules in KOA chondrocytes and interleukin (IL)-1β-stimulated chondrocytes. The correlation between the circ_0007482 expression and inflammatory factors was analyzed by the Pearson method. KOA cell model was established using IL-1β for 24 hours. The proliferation activity of chondrocytes was evaluated by CCK-8 assay, and cell apoptosis rate was assessed by flow cytometry. The downstream miRNA of circ_0007482 was validated using dual-luciferase reporter assay.
The circ_0007482 expression was elevated in both KOA cartilage tissues and IL-1β-treated chondrocytes and positively correlated with inflammatory factors expression. In comparison to the control group, IL-1β treatment diminished chondrocyte proliferation abilities and increased cell apoptosis and inflammatory factors IL-6, IL-8, and tumor necrosis factor (TNF)-α mRNA expression. Inhibition of circ_0007482 partially improved IL-1β-induced inflammatory reaction. Circ_0007482 could negatively regulate the expression of miR-558.
Interfering of circ_0007482 might partially promote cell proliferation and differentiation, while inhibit cell apoptosis to improve joint injury by regulating miR-558 in IL-1β-treated chondrocyte cell model.
膝骨关节炎（KOA）是一种复杂的退行性关节疾病，也是关节功能障碍的主要原因。本研究旨在探讨hsa_circ_0007482在KOA炎症、增殖、分化和凋亡中的作用。
采用实时定量聚合酶链反应（PCR）检测KOA软骨细胞和白细胞介素（IL）-1β刺激的软骨细胞中circ_0007482、炎症因子和分化相关分子的表达。采用Pearson法分析circ_0007482表达与炎症因子之间的相关性。用IL-1β处理24小时建立KOA细胞模型。采用CCK-8法评估软骨细胞的增殖活性，采用流式细胞术评估细胞凋亡率。采用双荧光素酶报告基因检测法验证circ_0007482的下游miRNA。
circ_0007482在KOA软骨组织和IL-1β处理的软骨细胞中均表达升高，且与炎症因子表达呈正相关。与对照组相比，IL-1β处理降低了软骨细胞的增殖能力，增加了细胞凋亡以及炎症因子IL-6、IL-8和肿瘤坏死因子（TNF）-α mRNA的表达。抑制circ_0007482可部分改善IL-1β诱导的炎症反应。Circ_0007482可负向调节miR-558的表达。
在IL-1β处理的软骨细胞模型中，干扰circ_0007482可能通过调节miR-558部分促进细胞增殖和分化，同时抑制细胞凋亡以改善关节损伤。
