环状 RNA 0002715 通过海绵吸附 miR-127-5p 调控 LXN 促进骨关节炎的发展。

Circ_0002715 promotes the development of osteoarthritis through regulating LXN by sponging miR-127-5p.

机构信息

Department of Rehabilitation, Chongqing Traditional Chinese Medicine Hospital, No. 6, Panxi Qizhi Road, Jiangbei District, Chongqing, 400021, China.

Department of Acupuncture, Chongqing Traditional Chinese Medicine Hospital, Chongqing, 400021, China.

出版信息

J Orthop Surg Res. 2023 Mar 22;18(1):230. doi: 10.1186/s13018-023-03638-3.

BACKGROUND

Our study aims to investigate the role and mechanism of circular RNA_0002715 (circ_0002715) in osteoarthritis (OA) progression.

METHODS

IL-1β-induced CHON-001 cells were used to mimic OA cell model. Circ_0002715, microRNA (miR)-127-5p and Latexin (LXN) expression was detected by quantitative real-time PCR. Cell functions were determined by MTT assay, flow cytometry and ELISA assay. Protein expression was examined by western blot.

RESULTS

Circ_0002715 was highly expressed in OA cartilage tissues. Circ_0002715 silencing inhibited inflammation, apoptosis, and ECM degradation in IL-1β-interfered CHON-001 cells. Circ_0002715 could sponge miR-127-5p, and miR-127-5p could target LXN. The effect of circ_0002715 down-regulation on chondrocyte injury was partially restored by miR-127-5p inhibitor. MiR-127-5p could suppress chondrocyte injury by inhibiting LXN expression.

CONCLUSION

Circ_0002715 might be a new therapeutic target for OA, which regulated miR-127-5p/LXN axis to promote IL-1β-induced chondrocyte injury.

背景

本研究旨在探讨环状 RNA_0002715（circ_0002715）在骨关节炎（OA）进展中的作用和机制。

方法

采用白细胞介素-1β（IL-1β）诱导的 CHON-001 细胞模拟 OA 细胞模型。采用实时定量 PCR 检测 circ_0002715、微小 RNA（miR）-127-5p 和 Latexin（LXN）的表达。通过 MTT 法、流式细胞术和 ELISA 法测定细胞功能。通过 Western blot 检测蛋白表达。

结果

circ_0002715 在 OA 软骨组织中高表达。circ_0002715 沉默抑制了 IL-1β 干预的 CHON-001 细胞中的炎症、凋亡和细胞外基质降解。circ_0002715 可以海绵吸附 miR-127-5p，miR-127-5p 可以靶向 LXN。circ_0002715 下调对软骨细胞损伤的作用部分被 miR-127-5p 抑制剂恢复。miR-127-5p 可以通过抑制 LXN 表达来抑制软骨细胞损伤。