Department of Rehabilitation, Chongqing Traditional Chinese Medicine Hospital, No. 6, Panxi Qizhi Road, Jiangbei District, Chongqing, 400021, China.
Department of Acupuncture, Chongqing Traditional Chinese Medicine Hospital, Chongqing, 400021, China.
J Orthop Surg Res. 2023 Mar 22;18(1):230. doi: 10.1186/s13018-023-03638-3.
Our study aims to investigate the role and mechanism of circular RNA_0002715 (circ_0002715) in osteoarthritis (OA) progression.
IL-1β-induced CHON-001 cells were used to mimic OA cell model. Circ_0002715, microRNA (miR)-127-5p and Latexin (LXN) expression was detected by quantitative real-time PCR. Cell functions were determined by MTT assay, flow cytometry and ELISA assay. Protein expression was examined by western blot.
Circ_0002715 was highly expressed in OA cartilage tissues. Circ_0002715 silencing inhibited inflammation, apoptosis, and ECM degradation in IL-1β-interfered CHON-001 cells. Circ_0002715 could sponge miR-127-5p, and miR-127-5p could target LXN. The effect of circ_0002715 down-regulation on chondrocyte injury was partially restored by miR-127-5p inhibitor. MiR-127-5p could suppress chondrocyte injury by inhibiting LXN expression.
Circ_0002715 might be a new therapeutic target for OA, which regulated miR-127-5p/LXN axis to promote IL-1β-induced chondrocyte injury.
本研究旨在探讨环状 RNA_0002715（circ_0002715）在骨关节炎（OA）进展中的作用和机制。
采用白细胞介素-1β（IL-1β）诱导的 CHON-001 细胞模拟 OA 细胞模型。采用实时定量 PCR 检测 circ_0002715、微小 RNA（miR）-127-5p 和 Latexin（LXN）的表达。通过 MTT 法、流式细胞术和 ELISA 法测定细胞功能。通过 Western blot 检测蛋白表达。
circ_0002715 在 OA 软骨组织中高表达。circ_0002715 沉默抑制了 IL-1β 干预的 CHON-001 细胞中的炎症、凋亡和细胞外基质降解。circ_0002715 可以海绵吸附 miR-127-5p，miR-127-5p 可以靶向 LXN。circ_0002715 下调对软骨细胞损伤的作用部分被 miR-127-5p 抑制剂恢复。miR-127-5p 可以通过抑制 LXN 表达来抑制软骨细胞损伤。
circ_0002715 可能是 OA 的一个新的治疗靶点，通过调节 miR-127-5p/LXN 轴促进 IL-1β 诱导的软骨细胞损伤。
