Graduate School of North China University of Technology, Tangshan, China.
Department of Respiratory and Critical Care Medicine, Emergency General Hospital, Beijing, China.
Microbiol Spectr. 2024 Jun 4;12(6):e0347223. doi: 10.1128/spectrum.03472-23. Epub 2024 May 15.
Malignant central airway stenosis is treated with airway stent placement, but post-placement microbial characteristics remain unclear. We studied microbial features in 60 patients post-stent placement, focusing on changes during granulation tissue proliferation. Samples were collected before stent ( = 29), after stent on day 3 ( = 20), and after granulation tissue formation (AS-GTF, = 43). Metagenomic sequencing showed significant respiratory tract microbiota changes with granulation tissue. The microbiota composition, dominated by , , and , was similar among the groups. At the species level, the AS-GTF group exhibited significant differences, with and enriched. Analysis based on tracheoesophageal fistula presence identified and as the main differential species, enriched in the fistula subgroup. Viral and fungal detection showed and as the main species, respectively. These findings highlight microbiota changes after stent placement, potentially associated with granulation tissue proliferation, informing stent placement therapy and anti-infective treatment optimization.
Malignant central airway stenosis is a life-threatening condition that can be effectively treated with airway stent placement. However, despite its clinical importance, the microbial characteristics of the respiratory tract following stent insertion remain poorly understood. This study addresses this gap by investigating the microbial features in patients with malignant central airway stenosis after stent placement, with a specific focus on microbial changes during granulation tissue proliferation. The findings reveal significant alterations in the diversity and structure of the respiratory tract microbiota following the placement of malignant central airway stents. Notably, certain bacterial species, including and , exhibit distinct patterns in the after-stent granulation tissue formation group. Additionally, the presence of tracheoesophageal fistula further influences the microbial composition. These insights provide valuable references for optimizing stent placement therapy and enhancing clinical anti-infective strategies.
恶性中央气道狭窄是一种危及生命的疾病，可以通过气道支架置入术有效治疗。然而，尽管其具有重要的临床意义，但支架置入后呼吸道的微生物特征仍知之甚少。本研究通过调查恶性中央气道狭窄患者支架置入后的微生物特征，特别关注肉芽组织增生过程中的微生物变化，解决了这一空白。
支架置入后，呼吸道微生物多样性和结构发生显著改变。值得注意的是，某些细菌物种，包括 和 ，在支架置入后肉芽组织形成组中表现出明显的变化模式。此外，气管食管瘘的存在进一步影响了微生物组成。这些发现为优化支架置入治疗和加强临床抗感染策略提供了有价值的参考。
