Hu Kevin, O'Neil Thomas, Canete Nicolas, Baharlou Heeva, Harman Andrew
Centre for Virus Research, The Westmead Institute for Medical Research, Westmead, New South Wales, Australia.
School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, New South Wales, Australia.
Cytometry A. 2024 Jul;105(7):488-492. doi: 10.1002/cyto.a.24847. Epub 2024 May 15.
We introduce a 35-marker imaging mass cytometry (IMC) panel for a detailed examination of immune cell populations and HIV RNA in formalin fixed paraffin embedded (FFPE) human intestinal tissue. The panel has broad cell type coverage and particularly excels in delineating subsets of mononuclear phagocytes and T cells. Markers for key tissue structures are included, enabling identification of epithelium, blood vessels, lymphatics, and musculature. The described method for HIV RNA detection can be generalized to other low abundance RNA targets, whether endogenous or pathogen derived. As such, the panel presented here is useful for high parameter spatial mapping of intestinal immune cells and their interactions with pathogens such as HIV.
我们引入了一个包含35种标志物的成像质谱流式细胞术（IMC）检测板，用于在福尔马林固定石蜡包埋（FFPE）的人体肠道组织中详细检测免疫细胞群体和HIV RNA。该检测板具有广泛的细胞类型覆盖范围，尤其擅长区分单核吞噬细胞和T细胞亚群。其中包含了关键组织结构的标志物，能够识别上皮、血管、淋巴管和肌肉组织。所描述的HIV RNA检测方法可推广至其他低丰度RNA靶点，无论是内源性的还是病原体衍生的。因此，本文介绍的检测板对于肠道免疫细胞及其与HIV等病原体相互作用的高参数空间图谱绘制很有用。
