Mater Research Institute - University of Queensland, TRI Building, Woolloongabba, QLD 4102, Australia.
Queensland Brain Institute, University of Queensland, Brisbane, QLD 4072, Australia.
Biochem Soc Trans. 2024 Jun 26;52(3):1431-1447. doi: 10.1042/BST20231322.
Whole genome sequencing of viruses provides high-resolution molecular insights, enhancing our understanding of viral genome function and phylogeny. Beyond fundamental research, viral sequencing is increasingly vital for pathogen surveillance, epidemiology, and clinical applications. As sequencing methods rapidly evolve, the diversity of viral genomics applications and catalogued genomes continues to expand. Advances in long-read, single molecule, real-time sequencing methodologies present opportunities to sequence contiguous, haplotype resolved viral genomes in a range of research and applied settings. Here we present an overview of nucleic acid sequencing methods and their applications in studying viral genomes. We emphasise the advantages of different viral sequencing approaches, with a particular focus on the benefits of third-generation sequencing technologies in elucidating viral evolution, transmission networks, and pathogenesis.
病毒的全基因组测序提供了高分辨率的分子见解，增强了我们对病毒基因组功能和系统发育的理解。除了基础研究外，病毒测序对于病原体监测、流行病学和临床应用也变得越来越重要。随着测序方法的快速发展，病毒基因组学应用和编目的基因组的多样性不断扩大。长读长、单分子、实时测序方法的进步为在一系列研究和应用环境中连续、单倍型解析病毒基因组提供了机会。本文概述了核酸测序方法及其在研究病毒基因组中的应用。我们强调了不同病毒测序方法的优势，特别关注第三代测序技术在阐明病毒进化、传播网络和发病机制方面的优势。
