Xie Tengfei, Wu Qingping, Xu Xiaoke, Zhang Jumei, Guo Weipeng
School of Bioscience and Bioengineering, South China University of Technology, No. 382 Waihuandong Road, Guangzhou 510006, PR China Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, No. 100 Central Xianlie Road, Guangzhou 510070, PR China.
Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, No. 100 Central Xianlie Road, Guangzhou 510070, PR China
FEMS Microbiol Lett. 2015 Nov;362(22). doi: 10.1093/femsle/fnv178. Epub 2015 Sep 30.
Vibrio parahaemolyticus is a common foodborne pathogen in aquatic products. To investigate the prevalence of Vibrio parahaemolyticus in aquatic products in South China, 224 samples were collected from markets in four provinces (11 cities) from May 2013 to January 2014. One hundred and fifty isolates were isolated from 98 samples. All isolates were analyzed for the presence of thermostable direct haemolysin (TDH) and TDH-related haemolysin (TRH) by PCR, antibiotic susceptibility analysis by disk diffusion method, serotyping by multiplex PCR and molecular typing by enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) typing. Although all 150 isolates were negative for tdh, 61 strains were trh positive (40.67%). Antimicrobial susceptibility results indicated that most strains were resistant to streptomycin (88.67%), cefazolin (66.00%) and ampicillin (62.67%). All strains were susceptible to chloramphenicol. Forty percent of all isolates were O2 type. The 150 isolates were grouped into three clusters by ERIC-PCR typing. The results demonstrated the presence of V. parahaemolyticus in aquatic products from the retail market and this methodology can be used for microbiological risk assessment in China.
副溶血性弧菌是水产品中常见的食源性病原体。为调查中国南方水产品中副溶血性弧菌的流行情况，于2013年5月至2014年1月从四个省份（11个城市）的市场采集了224份样本。从98份样本中分离出150株菌株。通过PCR分析所有分离株中耐热直接溶血素（TDH）和TDH相关溶血素（TRH）的存在情况，采用纸片扩散法进行药敏分析，通过多重PCR进行血清分型，并通过肠杆菌重复基因间共有序列PCR（ERIC-PCR）分型进行分子分型。尽管所有150株分离株的tdh均为阴性，但61株菌株trh呈阳性（40.67%）。药敏结果表明，大多数菌株对链霉素（88.67%）、头孢唑林（66.00%）和氨苄西林（62.67%）耐药。所有菌株对氯霉素敏感。所有分离株的40%为O2型。通过ERIC-PCR分型将150株分离株分为三个簇。结果表明零售市场的水产品中存在副溶血性弧菌，该方法可用于中国的微生物风险评估。
