Yan Wei, Chen Liping, Ji Lei, Yuan Rui, Dong Fenfen, Zhang Peng
Huzhou Center for Disease Control and Prevention, 999 Changxing Road, Huzhou 313000, China.
Foods. 2025 Jul 15;14(14):2481. doi: 10.3390/foods14142481.
is the leading bacterial cause of gastroenteritis associated with aquatic food consumption globally. This study aimed to determine the prevalence of in aquatic foods from Huzhou and to identify the serotypes, antimicrobial resistance, virulence factors, and genetic relatedness of the strains. A total of 306 isolates were detected from 1314 aquatic food samples from 2022 to 2024. The results indicated that the most prevalent serotypes were O1:KUT (17.0%), O2:K28 (13.7%), and O2:KUT (13.1%). Multilocus sequence typing analysis divided the 306 isolates into 175 sequence types (STs), and the predominant sequence type was ST864 (3.3%). Antimicrobial susceptibility tests showed that 2.6% of isolates were multidrug resistant. High resistance was observed to ampicillin (64.7%) and streptomycin (44.4%). A total of seven antimicrobial categories of resistance genes were identified, and the resistance gene was detected in all isolates. The virulence genes and were found in 16 (5.2%) and 12 (3.9%) isolates, respectively. In addition, we observed that all the 306 isolates encode type III secretion systems 1. The phylogenomic analysis based on the whole-genome sequence revealed that the 306 isolates were divided into four clusters. Our findings broaden perspectives on genetic diversity and enhance our ability to assess the potential risks of its spread.
是全球与食用水产食品相关的肠胃炎的主要细菌病因。本研究旨在确定湖州水产食品中该菌的流行情况，并鉴定菌株的血清型、抗菌药物耐药性、毒力因子和遗传相关性。2022年至2024年，从1314份水产食品样本中共检测到306株分离株。结果表明，最常见的血清型为O1:KUT（17.0%）、O2:K28（13.7%）和O2:KUT（13.1%）。多位点序列分型分析将306株分离株分为175个序列型（STs），主要序列型为ST864（3.3%）。抗菌药物敏感性试验表明，2.6%的分离株对多种药物耐药。观察到对氨苄西林（64.7%）和链霉素（44.4%）的高耐药性。共鉴定出七类抗菌药物耐药基因，所有分离株均检测到耐药基因。分别在16株（5.2%）和12株（3.9%）分离株中发现了毒力基因和。此外，我们观察到所有306株该菌分离株均编码1型III分泌系统。基于全基因组序列的系统发育分析表明，306株分离株分为四个簇。我们的研究结果拓宽了对该菌遗传多样性的认识，并提高了我们评估其传播潜在风险的能力。
