Center for Smart Manufacturing, Division of Integrative Systems and Design, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, 999077, China.
Center for Smart Manufacturing, Division of Integrative Systems and Design, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, 999077, China; Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Clear Water Bay, Kowloon, Hong Kong SAR, 999077, China.
Biosens Bioelectron. 2024 Sep 1;259:116386. doi: 10.1016/j.bios.2024.116386. Epub 2024 May 13.
Faced with the increasing prevalence of chronic kidney disease (CKD), portable monitoring of CKD-related biomarkers such as potassium ion (K), creatinine (Cre), and lactic acid (Lac) levels in sweat has shown tremendous potential for early diagnosis. However, a rapidly manufacturable portable device integrating multiple CKD-related biomarker sensors for ease of sweat testing use has yet to be reported. Here, a portable electrochemical sensor integrated with multifunctional laser-induced graphene (LIG) circuits and laser-printed nanomaterials based working electrodes fabricated by fully automatic laser manufacturing is proposed for non-invasive human kidney function monitoring. The sensor comprises a two-electrode LIG circuit for K sensing, a three-electrode LIG circuit with a Kelvin compensating connection for Cre and Lac sensing, and a printed circuit board based portable electrochemical workstation. The working electrodes containing Cu and CuO nanoparticles fabricated by two-step laser printing show good sensitivity and selectivity toward Cre and Lac sensing. The sensor circuits are fabricated by generating a hydrophilic-hydrophobic interface on a patterned LIG through laser. This sensor recruited rapid laser manufacturing and integrated with multifunctional LIG circuits and laser-printed nanomaterials based working electrodes, which is a potential kidney function monitoring solution for healthy people and kidney disease patients.
面对慢性肾脏病（CKD）发病率的不断上升，便携式监测汗液中钾离子（K）、肌酸酐（Cre）和乳酸（Lac）等与 CKD 相关的生物标志物，对于早期诊断具有巨大的潜力。然而，一种可快速制造的便携式设备，能够集成多个与 CKD 相关的生物标志物传感器，以便于汗液检测的使用，这方面仍有待报道。在这里，我们提出了一种集成多功能激光诱导石墨烯（LIG）电路和基于激光打印纳米材料的工作电极的便携式电化学传感器，用于非侵入式人体肾功能监测。该传感器包括一个用于 K 感应的双电极 LIG 电路、一个带有 Kelvin 补偿连接的三电极 LIG 电路，用于 Cre 和 Lac 感应，以及一个基于印刷电路板的便携式电化学工作站。通过两步激光打印制备的含有 Cu 和 CuO 纳米颗粒的工作电极对 Cre 和 Lac 感应表现出良好的灵敏度和选择性。通过激光在图案化的 LIG 上产生亲水-疏水界面来制造传感器电路。该传感器采用快速激光制造技术，集成了多功能 LIG 电路和基于激光打印纳米材料的工作电极，有望成为健康人群和肾病患者的肾功能监测解决方案。
