Research Center on Smart Manufacturing, Division of Integrative Systems and Design, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, 999077, P. R. China.
Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Clear Water Bay, Kowloon, Hong Kong SAR, 999077, P. R. China.
Small Methods. 2024 Mar;8(3):e2301184. doi: 10.1002/smtd.202301184. Epub 2023 Nov 27.
A portable sweat urea sensing system is a promising solution to satisfy the booming requirement of kidney function tele-monitoring. However, the complicated manufacturing route and the cumbersome electrochemical testing system still need to be improved to develop the urea point-of-care testing (POCT) and tele-monitoring devices. Here, a universal technical route based on a high-throughput automatic laser printing strategy for fabricating the portable integrated urea monitoring system is proposed. This integrated system includes a high-performance laser-printed urea sensing electrode, a planar three-electrode system, and a self-developed wireless mini-electrochemical workstation. A precursor donor layer is activated by laser scribing and in situ transferred into functional nanoparticles for the drop-on-demand printing of the urea sensing electrode. The obtained electrodes show high sensitivity, low detection limit, fast response time, high selectivity, good average recovery, and long-term stability for urea sensing. Additionally, a laser-induced graphene circuit-based miniature planar three-electrode system and a wireless mini-electrochemical workstation are designed for sensing data collection and transmitting, achieving real-time urea POCT and tele-monitoring. This scalable method provides a universal solution for high-throughput and ultra-fast fabrication of urea-sensing electrodes. The portable integrated urea monitoring system is a competitive option to achieve cost-effective POCT and tele-monitoring for kidney function.
一种便携式汗液尿素传感系统是满足肾功能远程监测需求的有前途的解决方案。然而，复杂的制造路线和繁琐的电化学测试系统仍然需要改进，以开发尿素即时检测（POCT）和远程监测设备。在这里，提出了一种基于高通量自动激光打印策略的通用技术路线，用于制造便携式集成尿素监测系统。该集成系统包括高性能激光打印尿素传感电极、平面三电极系统和自主开发的无线微型电化学工作站。前体供体层通过激光划线激活，并原位转化为功能性纳米颗粒，用于按需打印尿素传感电极。所获得的电极对尿素传感表现出高灵敏度、低检测限、快速响应时间、高选择性、良好的平均回收率和长期稳定性。此外，还设计了基于激光诱导石墨烯电路的微型平面三电极系统和无线微型电化学工作站，用于传感数据的采集和传输，实现实时尿素 POCT 和远程监测。这种可扩展的方法为高通量和超快尿素传感电极制造提供了通用解决方案。便携式集成尿素监测系统是实现具有成本效益的肾功能 POCT 和远程监测的有竞争力的选择。
