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推断基于B细胞衍生T细胞受体诱导的多表位肠道病毒71型候选疫苗：一种反向疫苗学方法。

Inferring B-cell derived T-cell receptor induced multi-epitope-based vaccine candidate against enterovirus 71: a reverse vaccinology approach.

作者信息

Swain Subrat Kumar, Panda Subhasmita, Sahu Basanta Pravas, Mahapatra Soumya Ranjan, Dey Jyotirmayee, Sarangi Rachita, Misra Namrata

机构信息

Department of Medical Research, IMS and SUM Hospital, Siksha "O" Anusandhan Deemed to be University, Bhubaneswar, India.

Department of Pediatrics, IMS and SUM Hospital, Siksha "O" Anusandhan Deemed to be University, Bhubaneswar, India.

出版信息

Clin Exp Vaccine Res. 2024 Apr;13(2):132-145. doi: 10.7774/cevr.2024.13.2.132. Epub 2024 Apr 30.

DOI:10.7774/cevr.2024.13.2.132
PMID:38752008
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11091429/
Abstract

PURPOSE

Enterovirus 71, a pathogen that causes hand-foot and mouth disease (HFMD) is currently regarded as an increasing neurotropic virus in Asia and can cause severe complications in pediatric patients with blister-like sores or rashes on the hand, feet, and mouth. Notwithstanding the significant burden of the disease, no authorized vaccine is available. Previously identified attenuated and inactivated vaccines are worthless over time owing to changes in the viral genome.

MATERIALS AND METHODS

A novel vaccine construct using B-cell derived T-cell epitopes from the virulent polyprotein found the induction of possible immune response. In order to boost the immune system, a beta-defensin 1 preproprotein adjuvant with EAAAK linker was added at the N-terminal end of the vaccine sequence.

RESULTS

The immunogenicity of the designed, refined, and verified prospective three-dimensional-structure of the multi-epitope vaccine was found to be quite high, exhibiting non-allergenic and antigenic properties. The vaccine candidates bound to toll-like receptor 3 in a molecular docking analysis, and the efficacy of the potential vaccine to generate a strong immune response was assessed through immunological simulation.

CONCLUSION

Computational analysis has shown that the proposed multi-epitope vaccine is possibly safe for use in humans and can elicit an immune response.

摘要

目的

肠道病毒71型是一种导致手足口病（HFMD）的病原体，目前在亚洲被视为一种嗜神经病毒，可在手部、足部和口腔出现水泡样溃疡或皮疹的儿科患者中引发严重并发症。尽管该疾病负担沉重，但尚无获批疫苗。由于病毒基因组的变化，先前鉴定的减毒和灭活疫苗随着时间推移已毫无价值。

材料与方法

一种使用来自强毒株多聚蛋白的B细胞衍生T细胞表位的新型疫苗构建体发现可诱导可能的免疫反应。为增强免疫系统，在疫苗序列的N末端添加了带有EAAAK接头的β-防御素1前原蛋白佐剂。

结果

发现设计、优化并验证的多表位疫苗预期三维结构的免疫原性相当高，具有非致敏性和抗原性。在分子对接分析中，候选疫苗与Toll样受体3结合，并通过免疫模拟评估了潜在疫苗产生强烈免疫反应的效力。

结论

计算分析表明，所提出的多表位疫苗可能对人类使用安全，并可引发免疫反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d328/11091429/510f9e556564/cevr-13-132-g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d328/11091429/75b9381b4b07/cevr-13-132-g007.jpg
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