sp. nov., isolated from marine sediment.

A novel actinobacterium, strain ZYX-F-186, was isolated from marine sediment sampled on Yongxing Island, Hainan Province, PR China. Based on the results of 16S rRNA gene sequence analysis, strain ZYX-F-186 belongs to the genus , with high similarity to KK1-3 (98.3 %), K11-0047 (98.1 %), K09-0627 (98.1 %), K11-0057 (97.9 %), K07-0523 (97.7 %), and RD004123 (97.7 %). Phylogenetic analysis of 16S rRNA gene sequences showed that the strain formed a single subclade in the genus . The novel isolate contained -diaminopimelic acid, d-glutamic acid, glycine, d-alanine, and l-lysine in the cell wall. The whole-cell sugars were xylose, arabinose, ribose, and rhamnose. The predominant menaquinones were MK-9(H), MK-9(H), and MK-9(H). The characteristic phospholipids were phosphatidylethanolamine, phosphatidylinositol, phosphatidylmethylethanolamine, phosphatidylglycerol, and an unknown phospholipid. The major fatty acids (>5 %) were iso-C, anteiso-C, and iso-C. Genome sequencing showed a DNA G+C content of 71.9 mol%. Low average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity values demonstrated that strain ZYX-F-186 could be readily distinguished from its closely related species. Based on its phylogenetic, chemotaxonomic, and physiological characteristics, strain ZYX-F-186 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ZYX-F-186 (=CGMCC 4.8025=CCTCC AA 2023025=JCM 36507).