School of Life Sciences, Northwestern Polytechnical University, Xi'an, 710072, PR China; Engineering Research Center of Chinese Ministry of Education for Biological Diagnosis, Treatment and Protection Technology and Equipment, Xi'an, 710072, PR China; Center for Advanced Biomaterials and Technology Transformation, Suzhou Ningrao Biotechnology Co., Ltd, Suzhou, 215000, PR China.
School of Life Sciences, Northwestern Polytechnical University, Xi'an, 710072, PR China; Engineering Research Center of Chinese Ministry of Education for Biological Diagnosis, Treatment and Protection Technology and Equipment, Xi'an, 710072, PR China.
Biosens Bioelectron. 2024 Sep 1;259:116355. doi: 10.1016/j.bios.2024.116355. Epub 2024 May 1.
Coronavirus disease 2019 (COVID-19) is a highly contagious illness caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), resulting in a global health crisis. The primary diagnostic method for COVID-19 is quantitative reverse transcription PCR, which is time-consuming and requires expensive instrumentation. Here, we developed an electrochemical biosensor for detecting SARS-CoV-2 biomarkers using a 3D porous polyacrylamide/polyaniline hydrogel (PPG) electrode prepared by UV photopolymerization and in situ polymerization. The electrochemical immunosensor for detecting SARS-CoV-2 N protein via the immune sandwich principle demonstrated a lower detection limit of 42 pg/mL and comparable specificity to a commercial enzyme-linked immunosorbent assay, which was additionally validated in pseudoviruses. The electrochemical sensor for hydrogen peroxide showed a low detection limit of 0.5 μM and excellent selectivity, which was further confirmed in cancer cells under oxidative stress. The biomarkers of SARS-CoV-2 were successfully detected due to the signal amplification capability provided by 3D porous electrodes and the high sensitivity of the antigen-antibody specific binding. This study introduces a novel three-dimensional electrode with great potential for the early detection of SARS-CoV-2.
新型冠状病毒病（COVID-19）是由严重急性呼吸系统综合征冠状病毒 2（SARS-CoV-2）引起的高度传染性疾病，导致了全球卫生危机。COVID-19 的主要诊断方法是定量逆转录聚合酶链反应（qRT-PCR），该方法既耗时又需要昂贵的仪器。在这里，我们使用通过紫外光聚合和原位聚合制备的 3D 多孔聚丙烯酰胺/聚苯胺水凝胶（PPG）电极，开发了一种用于检测 SARS-CoV-2 生物标志物的电化学生物传感器。通过免疫夹心原理检测 SARS-CoV-2 N 蛋白的电化学免疫传感器显示出 42pg/mL 的较低检测限和与商业酶联免疫吸附测定相当的特异性，该传感器在假病毒中得到了进一步验证。用于检测过氧化氢的电化学传感器显示出 0.5μM 的低检测限和出色的选择性，在氧化应激下的癌细胞中得到了进一步证实。由于 3D 多孔电极提供的信号放大能力和抗原-抗体特异性结合的高灵敏度，成功检测到了 SARS-CoV-2 的生物标志物。本研究介绍了一种新型的三维电极，具有用于 SARS-CoV-2 早期检测的巨大潜力。
