School of Civil Engineering and Architecture, Anhui University of Technology, Ma'anshan, 243032, China; Engineering Research Center of Biofilm Water Purification and Utilization Technology of Ministry of Education, Anhui University of Technology, Ma'anshan, 243032, China.
School of Civil Engineering and Architecture, Anhui University of Technology, Ma'anshan, 243032, China.
Environ Res. 2024 Jul 1;252(Pt 4):119151. doi: 10.1016/j.envres.2024.119151. Epub 2024 May 14.
The aim of this study was to assess effects of MnO addition (CK-0%, T1-2% and T2-5%) on humification and bacterial community during municipal sludge (MS) composting. The results suggested that MnO addition inhibited the growth of Nitrospira but stimulated Nonomuraea, Actinomadura, Streptomyces and Thermopolyspora, facilitating the lignocellulose degradation and humification with the increase in organic matter degradation by 13.8%-19.2% and humic acid content by 10.9%-20.6%. Compared to CK, the abundances of exoglucanase (EC:3.2.1.91), endo-1,4-beta-xylanase (EC:3.2.1.136) and endomannanase (EC:3.2.1.78) increased by 88-99, 52-66 and 4-15 folds, respectively. However, 5%-MnO induced the enrichment of Mizugakiibacter that harms the environment of agricultural production. The addition of 2%-MnO was recommended for MS composting. Furthermore, metabolic function analysis indicated that MnO addition altered amino acid and carbohydrate metabolism, especially enhancing propanoate metabolism and butanoate metabolism but inhibiting citrate cycle. Structural equation modeling revealed that Nonomuraea and Actinomadura were the main drivers for lignocellulose degradation. This study provided theoretical guidance in regulating humification via MnO for MS composting.
本研究旨在评估 MnO 添加（CK-0%、T1-2%和 T2-5%）对城市污泥（MS）堆肥过程中腐殖化和细菌群落的影响。结果表明，MnO 添加抑制了 Nitrospira 的生长，但刺激了 Nonomuraea、Actinomadura、Streptomyces 和 Thermopolyspora 的生长，促进了木质纤维素的降解和腐殖化，使有机物降解率提高了 13.8%-19.2%，腐殖酸含量提高了 10.9%-20.6%。与 CK 相比，外切葡聚糖酶（EC：3.2.1.91）、内切 1,4-β-木聚糖酶（EC：3.2.1.136）和内切甘露聚糖酶（EC：3.2.1.78）的丰度分别提高了 88-99、52-66 和 4-15 倍。然而，5%-MnO 诱导了对农业生产环境有害的 Mizugakiibacter 的富集。建议在 MS 堆肥中添加 2%-MnO。此外，代谢功能分析表明，MnO 添加改变了氨基酸和碳水化合物代谢，特别是增强了丙酸代谢和丁酸代谢，但抑制了柠檬酸循环。结构方程模型表明，Nonomuraea 和 Actinomadura 是木质纤维素降解的主要驱动因素。本研究为通过 MnO 调节 MS 堆肥腐殖化提供了理论指导。
