Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, China; Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing 210037, China.
Int J Biol Macromol. 2024 Jun;270(Pt 2):132339. doi: 10.1016/j.ijbiomac.2024.132339. Epub 2024 May 14.
Xylan-type hemicellulose hydrolysis by an organic acid solution for the production of xylo-oligosaccharides (XOS) is efficient and eco-friendly, but the effects of different organic acids on XOS production from Toona sinensis branch (TB) biomass is limited. In this work, under the conditions of 170 °C for 60 min, 33.1 % and 38.7 % XOS yields were obtained from polysaccharides present in TB by 2 % lactic acid (LA) and 6 % propionic acid (PA), respectively. Then 77 % of the lignin was removed by hydrogen peroxide-acetic acid pretreatment system, and 39.5 % and 44.7 % XOS yield were obtained from polysaccharides in delignification TB by 2 % LA and 6 % PA, respectively. It was found that PA hydrolysis, especially from delignified TB, resulted in higher XOS yield and purity compared to LA hydrolysis. Moreover, the content of byproducts (xylose, hydroxymethyl-furfural and furfural) in PA hydrolysate was lower. Following the hydrolysis process, the simultaneous saccharification and fermentation of the TB solid residue achieved an ethanol yield of 71.5 %. This work proposed an integrated process to preferentially convert the TB hemicellulose into valuable XOS and then convert the cellulose into ethanol. This process had the advantages of eliminating the need for isolation and purification of xylan, and the potential to obtain multiple products from the same raw material.
用有机酸溶液水解木聚糖型半纤维素生产木二糖（XOS）既高效又环保，但不同有机酸对香椿树枝（TB）生物质生产 XOS 的影响有限。在 170°C 下反应 60min 的条件下，2%乳酸（LA）和 6%丙酸（PA）分别从 TB 中的多糖中得到 33.1%和 38.7%的 XOS 得率。然后通过过氧化氢-乙酸预处理体系去除 77%的木质素，从脱木质素的 TB 中的多糖中分别得到 2%LA 和 6%PA 水解的 39.5%和 44.7%的 XOS 得率。结果表明，PA 水解，特别是从脱木质素的 TB 中，与 LA 水解相比，得到了更高的 XOS 得率和纯度。此外，PA 水解产物（木糖、羟甲基糠醛和糠醛）的含量较低。在水解过程之后，TB 固体残渣的同步糖化和发酵实现了 71.5%的乙醇得率。本工作提出了一种集成工艺，优先将 TB 半纤维素转化为有价值的 XOS，然后将纤维素转化为乙醇。该过程具有无需分离和纯化木聚糖的优势，并且有可能从同一种原料中获得多种产品。
