Ishizaka Y, Motoyoshi K, Shionoya S, Ikeda K, Hatake K, Saito M, Takaku F, Miura Y
Exp Hematol. 1985 Aug;13(7):591-6.
Sera obtained from seven normal volunteers and 11 patients with aplastic anemia were assayed for two types of colony-stimulating activity (CSA) using monolayer agar cultures containing human unfractionated and monocyte-depleted bone marrow cells. Sera obtained from normal volunteers had low CSA for unfractionated bone marrow cells and no CSA for monocyte-depleted bone marrow cells. On the other hand, sera obtained from patients with aplastic anemia (patients' sera) had moderate CSA for both unfractionated and monocyte-depleted bone marrow cells. The patients' serum CSA titer for unfractionated bone marrow cells rose markedly with the addition of a small amount of diluted control rabbit serum (control serum) into the CSA assay system, while it did not rise with addition of the same amount of diluted rabbit antiserum against partially purified human urinary colony-stimulating factor (CSF) (antiserum). The patients' serum CSA titer for monocyte-depleted human bone marrow cells rose with the addition of control serum as well as antiserum. These findings indicate that the addition of rabbit serum enhances colony formation by human bone marrow cells in the presence of aplastic anemia sera as a source of CSA, and that sera of patients with aplastic anemia contain two antigenically different types of CSA: one that is active on human unfractionated bone marrow cells and partially neutralized by the addition of antiserum, and another that is active on human monocyte-depleted bone marrow cells and is not neutralized by the addition of antiserum.
从7名正常志愿者和11名再生障碍性贫血患者身上获取血清，使用含有未分级人骨髓细胞和单核细胞去除的骨髓细胞的单层琼脂培养物，检测两种集落刺激活性（CSA）。正常志愿者的血清对未分级骨髓细胞的CSA较低，对单核细胞去除的骨髓细胞无CSA。另一方面，再生障碍性贫血患者的血清（患者血清）对未分级和单核细胞去除的骨髓细胞均有中度CSA。在CSA检测系统中加入少量稀释的对照兔血清（对照血清）后，患者血清对未分级骨髓细胞的CSA滴度显著升高，而加入等量稀释的抗部分纯化人尿集落刺激因子（CSF）的兔抗血清（抗血清）后则未升高。加入对照血清和抗血清后，患者血清对单核细胞去除的人骨髓细胞的CSA滴度均升高。这些发现表明，在存在作为CSA来源的再生障碍性贫血血清的情况下，加入兔血清可增强人骨髓细胞的集落形成，并且再生障碍性贫血患者的血清含有两种抗原性不同的CSA类型：一种对未分级人骨髓细胞有活性，加入抗血清后可部分中和；另一种对单核细胞去除的人骨髓细胞有活性，加入抗血清后不能中和。
