眼后段儿茶酚胺的定量分析与免疫组织化学

Quantitation and immunohistochemistry of catecholamines in the posterior segment of the eye.

作者信息

Ehinger B, Steinbusch H W

出版信息

Histochemistry. 1985;82(5):435-9. doi: 10.1007/BF02450478.

Abstract

Dopamine, noradrenaline, and adrenaline were assayed with HPLC in the light adapted retinae of carp, frog, chicken, pigeon, rat, guinea-pig, rabbit, cat, pig and cow. Dopamine varied from 0.6 to 2.6 nmol/g wet weight and was not influenced by sympathectomy. The dopamine figures agree with previously published results. Noradrenaline concentrations varied from not detectable to 0.06 nmol/g wet weight in different species. Homolateral sympathectomy significantly decreased the noradrenaline figure in rabbits. There are no previous figures for noradrenaline for most of the species. Adrenaline was not detected in any species. Immunohistochemical analysis showed noradrenaline to be present in choroidal nerves, but noradrenaline immuno-reactivity was not seen in the retina (chicken, rat, guinea-pig, rabbit, cat, cow). It is concluded that dopamine is the major catecholamine in the retina. Noradrenaline was found present only in minute amounts in the assays, and much of its was likely to stem from sympathetic nerve fibres. The study did not demonstrate any noradrenergic neurons in the retina.

摘要

采用高效液相色谱法（HPLC）对鲤鱼、青蛙、鸡、鸽子、大鼠、豚鼠、兔子、猫、猪和牛的明适应视网膜中的多巴胺、去甲肾上腺素和肾上腺素进行了测定。多巴胺含量在0.6至2.6 nmol/g湿重之间，且不受交感神经切除术的影响。多巴胺的测定结果与先前发表的结果一致。不同物种的去甲肾上腺素浓度在未检测到至0.06 nmol/g湿重之间变化。同侧交感神经切除术显著降低了兔子体内的去甲肾上腺素含量。大多数物种之前没有去甲肾上腺素的相关数据。在任何物种中均未检测到肾上腺素。免疫组织化学分析显示，脉络膜神经中存在去甲肾上腺素，但在视网膜（鸡、大鼠、豚鼠、兔子、猫、牛）中未观察到去甲肾上腺素免疫反应性。得出的结论是，多巴胺是视网膜中的主要儿茶酚胺。在测定中发现去甲肾上腺素仅以微量存在，其大部分可能来源于交感神经纤维。该研究未在视网膜中发现任何去甲肾上腺素能神经元。