25-羟基维生素D血浆浓度的年龄依赖性变化可能会使未成熟猫的维生素D状态评估复杂化。
Age-dependent changes in plasma concentrations of 25-hydroxyvitamin D may complicate vitamin D status assessment of immature cats.
作者信息
Backus Robert C, Ueda Devon C
机构信息
Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, MO, United States.
出版信息
Front Vet Sci. 2024 May 2;11:1365204. doi: 10.3389/fvets.2024.1365204. eCollection 2024.
BACKGROUND
Vitamin D deficiency and excess in clinically presented cats conventionally is diagnosed by comparison of patient plasma 25-hydroxyvitamin D (25 (OH)D) concentration with plasma reference intervals determined in healthy adult cats. For immature cats, validity of this vitamin D status assessment method is uncertain.
OBJECTIVE
The overall objective was determination of whether plasma concentration of 25 (OH) D and other vitamin D metabolites in immature cats markedly change with developmental age as has been reported in other species.
METHODS
Four male and 4 female domestic short-hair kittens from weaning were continuously presented a single nutritionally adequate growth-diet. Concentrations of 25 (OH) D and 24,25-dihydroxyvitamin D (24,25 (OH)D), and calcitriol were quantified in plasma of jugular venous blood collected at 12, 15, 18, and 21 weeks and 1 year of age. Plasma was liquid and solid-phase extracted and fractionation by normal-phase HPLC, and 25 (OH) D and 24,25 OH)D quantified by reverse-phase HPLC-UV and calcitriol by RIA.
RESULTS
Plasma 3-epi-25 (OH) D and 25 (OH) D concentrations increased ( < 0.001) with age so that by study end the concentrations rose by 1-and 2-fold, respectively. Concentrations of 3-epi-25 (OH) D relative to 25 (OH) D were 30% at 12 weeks and 20% at 1 year. Between ages 12 and 21 weeks, rises in 25 (OH) D concentration were positively correlated with body weight gains ( = 0.952, < 0.001) and 24,25 (OH)D concentrations were consistently greater than 25 (OH) D concentrations ( < 0.001). At 1 year of age, concentrations of 24,25 (OH)D declined below those of 25 (OH) D and 3-epi-24,25 (OH)D consistency occurred in low concentrations. Vitamin D metabolites and sex differences in metabolite concentrations were not observed.
CONCLUSION
Reliance on quantification of plasma 25 (OH) D concentration for vitamin D status assessment in kittens may be confounded by developmental changes in 25 (OH) D independent of vitamin D intake. High 24,25 (OH)D concentration and occurrence of 3-epi-25 (OH) D in plasma additionally may interfere with the quantification.
背景
临床上出现维生素D缺乏或过量的猫，传统上是通过将患猫血浆25-羟基维生素D（25（OH）D）浓度与健康成年猫血浆参考区间进行比较来诊断的。对于未成熟的猫，这种维生素D状态评估方法的有效性尚不确定。
目的
总体目标是确定未成熟猫血浆中25（OH）D和其他维生素D代谢产物的浓度是否会像其他物种那样随发育年龄发生显著变化。
方法
从断奶开始，连续给4只雄性和4只雌性家养短毛小猫提供单一营养充足的生长日粮。在12、15、18、21周龄和1岁时采集颈静脉血血浆，定量其中25（OH）D、24,25-二羟基维生素D（24,25（OH）D）和骨化三醇的浓度。血浆经液液和固相萃取，通过正相高效液相色谱进行分离，25（OH）D和24,25（OH）D通过反相高效液相色谱-紫外检测法定量，骨化三醇通过放射免疫分析法测定。
结果
血浆3-表-25（OH）D和25（OH）D浓度随年龄增加（<0.001），到研究结束时，浓度分别升高了1倍和2倍。3-表-25（OH）D相对于25（OH）D的浓度在12周时为30%，在1岁时为20%。在12至21周龄之间，25（OH）D浓度的升高与体重增加呈正相关（=0.952，<0.001），且24,25（OH）D浓度始终高于25（OH）D浓度（<0.001）。在1岁时，24,
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