Navarro R F, Jalkanen S T, Hsu M, Søenderstrup-Hansen G, Goronzy J, Weyand C, Fathman C G, Clayberger C, Krensky A M, Butcher E C
J Exp Med. 1985 Sep 1;162(3):1075-80. doi: 10.1084/jem.162.3.1075.
To function efficiently in vivo, lymphocytes must circulate from the blood into lymphoid tissues and other sites of immune reaction. Herein, we show that human cytotoxic and helper T cell clones and lines, maintained in vitro with IL-2, express the functional capacity to recognize and bind to high endothelial venules (HEV), a capacity essential for lymphocyte exit from the blood, and hence for normal lymphocyte trafficking. The expression of functional homing receptors distinguishes human T cell clones from their murine counterparts, which uniformly lack receptors for HEV and are unable to migrate normally from the blood in vivo. The results raise the possibility that human T cell clones may be more effective in mediating in vivo immune responses than is suggested by murine models.
为了在体内有效发挥功能，淋巴细胞必须从血液中循环至淋巴组织及其他免疫反应部位。在此，我们表明，在体外利用白细胞介素-2培养的人细胞毒性T细胞克隆及细胞系和辅助性T细胞克隆及细胞系，表达出识别并结合高内皮微静脉（HEV）的功能能力，这是淋巴细胞从血液中逸出所必需的能力，因此对于正常的淋巴细胞归巢至关重要。功能性归巢受体的表达使人T细胞克隆有别于小鼠T细胞克隆，后者普遍缺乏高内皮微静脉受体，并且在体内无法正常地从血液中迁移。这些结果增加了一种可能性，即人T细胞克隆在介导体内免疫反应方面可能比小鼠模型所显示的更有效。
