Sønderstrup-Hansen G, Petersen J, Claësson M H
Scand J Immunol. 1984 May;19(5):481-4. doi: 10.1111/j.1365-3083.1984.tb00957.x.
Twelve human T-cell lines were derived from cultures stimulated with phytohaemagglutinin (PHA) or with the recall antigen purified protein derivative (PPD), using the one-step agar colony method, followed by further expansion of individual T-cell colonies for at least 50 days. As judged by monoclonal antibodies, two of the PHA-derived T-cell lines carried the helper-inducer (H-I) phenotype and two the suppressor-cytotoxic (S-C) phenotype. Four PPD-derived cell lines displayed the H-I phenotype, and three of these proliferated specifically when challenged with the recall antigen, whereas the fourth did not. Four PPD-induced T-cell lines carried the S-C phenotype, and only one of these was antigen-specific. All cell lines with H-I phenotype displayed helper cell activity as determined by Ig secretion of B lymphocytes stimulated with a T-cell-dependent polyclonal B-cell activator. Cell lines with the S-C phenotype had no such helper activity and suppressed Ig responses in the presence of freshly isolated T cells. Thus, a good correlation between phenotypic markers and functions was found. T helper cells and T suppressor cells can be differentiated by means of both polyclonal and antigenic stimuli.
采用一步琼脂集落法，用植物血凝素（PHA）或回忆抗原纯化蛋白衍生物（PPD）刺激培养物，获得了12个人类T细胞系，随后将单个T细胞集落进一步扩增至少50天。通过单克隆抗体判断，两个PHA来源的T细胞系具有辅助诱导（H-I）表型，两个具有抑制细胞毒性（S-C）表型。四个PPD来源的细胞系表现出H-I表型，其中三个在用回忆抗原刺激时特异性增殖，而第四个则没有。四个PPD诱导的T细胞系具有S-C表型，其中只有一个是抗原特异性的。所有具有H-I表型的细胞系都表现出辅助细胞活性，这是通过用T细胞依赖性多克隆B细胞激活剂刺激B淋巴细胞分泌免疫球蛋白来确定的。具有S-C表型的细胞系没有这种辅助活性，并且在存在新鲜分离的T细胞的情况下抑制免疫球蛋白反应。因此，发现表型标志物与功能之间具有良好的相关性。T辅助细胞和T抑制细胞可以通过多克隆刺激和抗原刺激来区分。
