• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

利用预稳态通量比评估蛙皮上皮细胞中钠离子的转运途径。

Evaluation of transport pathways for Na+ across frog skin epithelium by means of presteady-state flux ratio.

作者信息

Eskesen K, Lim J J, Ussing H H

出版信息

J Membr Biol. 1985;86(2):99-104. doi: 10.1007/BF01870776.

DOI:10.1007/BF01870776
PMID:3875728
Abstract

A method is described that makes it possible to separate the sodium fluxes through the isolated frog skin into sets characteristic of the cellular and the paracellular pathway, respectively. If there are two significant pathways for the ion, and if they differ with respect to flux ratio as well as mean passage time, the flux ratios for the individual pathways can be obtained from a set of inward and outward tracer fluxes, covering the time from the addition of the tracers until the achievement of constant fluxes in both directions.

摘要

本文描述了一种方法,该方法能够将通过离体蛙皮的钠通量分别分离为细胞途径和细胞旁途径所特有的通量集。如果离子存在两条重要途径,并且它们在通量比以及平均通过时间方面存在差异,那么可以从一组内向和外向示踪剂通量中获得各个途径的通量比,这些通量涵盖了从添加示踪剂到双向达到恒定通量的时间段。

相似文献

1
Evaluation of transport pathways for Na+ across frog skin epithelium by means of presteady-state flux ratio.利用预稳态通量比评估蛙皮上皮细胞中钠离子的转运途径。
J Membr Biol. 1985;86(2):99-104. doi: 10.1007/BF01870776.
2
Determination of the electromotive force of active sodium transport in frog skin epithelium (Rana temporaria) from presteady-state flux ratio experiments.通过稳态前通量比实验测定蛙皮上皮组织(林蛙)中活性钠转运的电动势。
J Membr Biol. 1985;86(2):105-11. doi: 10.1007/BF01870777.
3
Transport pathways for Na+ and Br- (Cl-) in noradrenaline-stimulated frog skin (Rana temporaria).去甲肾上腺素刺激的蛙皮(林蛙)中Na⁺和Br⁻(Cl⁻)的转运途径
Acta Physiol Scand. 1989 Aug;136(4):535-46. doi: 10.1111/j.1748-1716.1989.tb08699.x.
4
Single-file diffusion through K+ channels in frog skin epithelium.
J Membr Biol. 1986;91(3):245-50. doi: 10.1007/BF01868817.
5
Effect of ouabain, amiloride, and antidiuretic hormone on the sodium-transport pool in isolated epithelia from frog skin (Rana temporaria).哇巴因、阿米洛利和抗利尿激素对林蛙(Rana temporaria)离体皮肤上皮钠转运池的影响。
J Membr Biol. 1982;65(3):221-6. doi: 10.1007/BF01869965.
6
Sodium recirculation and isotonic transport in toad small intestine.蟾蜍小肠中的钠再循环和等渗转运
J Membr Biol. 1999 Apr 1;168(3):241-51. doi: 10.1007/s002329900513.
7
Electrical rectification of the sodium flux across the apical barrier of frog skin epithelium.蛙皮上皮顶端屏障处钠通量的电整流作用。
Soc Gen Physiol Ser. 1981;36:15-30.
8
Coupled transepithelial sodium and potassium transport across isolated frog skin: effect of ouabain, amiloride and the polyene antibiotic filipin.跨分离蛙皮的上皮钠钾偶联转运:哇巴因、氨氯吡咪和多烯抗生素制霉菌素的作用
J Membr Biol. 1979 Dec 14;51(2):161-84. doi: 10.1007/BF01869167.
9
Current-voltage curve of sodium channels and concentration dependence of sodium permeability in frog skin.蛙皮中钠通道的电流-电压曲线及钠通透性的浓度依赖性
J Physiol. 1977 May;267(1):137-66. doi: 10.1113/jphysiol.1977.sp011805.
10
Saturation kinetics of sodium efflux across isolated frog skin.蛙离体皮肤钠外流的饱和动力学
Am J Physiol. 1976 Oct;231(4):995-1001. doi: 10.1152/ajplegacy.1976.231.4.995.

引用本文的文献

1
Fluid transport: a guide for the perplexed.液体运输:给困惑者的指南。
J Membr Biol. 2008 May;223(1):1-11. doi: 10.1007/s00232-007-9085-1. Epub 2008 Feb 8.
2
Determination of the electromotive force of active sodium transport in frog skin epithelium (Rana temporaria) from presteady-state flux ratio experiments.通过稳态前通量比实验测定蛙皮上皮组织(林蛙)中活性钠转运的电动势。
J Membr Biol. 1985;86(2):105-11. doi: 10.1007/BF01870777.
3
Single-file diffusion through K+ channels in frog skin epithelium.

本文引用的文献

1
DIFFUSION DELAY IN FROG SKIN CONNECTIVE TISSUE: A SOURCE OF ERROR IN TRACER INVESTIGATIONS.蛙皮结缔组织中的扩散延迟:示踪研究中的误差来源
Nature. 1964 Feb 29;201:932-3. doi: 10.1038/201932a0.
2
The origin of the short-circuit current in the adrenaline stimulated frog skin.肾上腺素刺激的蛙皮中短路电流的起源。
Acta Physiol Scand. 1952;27(1):38-48. doi: 10.1111/j.1748-1716.1953.tb00922.x.
3
The flux ratio equation under nonstationary conditions.非稳态条件下的通量比方程。
J Membr Biol. 1986;91(3):245-50. doi: 10.1007/BF01868817.
J Membr Biol. 1981;63(3):233-42. doi: 10.1007/BF01870984.
4
Analysis of presteady-state Na+ fluxes across the rabbit corneal endothelium.兔角膜内皮细胞跨膜钠离子通量的预稳态分析。
J Membr Biol. 1982;65(3):197-204. doi: 10.1007/BF01869963.
5
Enhanced sensitivity to stimulation of sodium transport and cyclic AMP by antidiuretic hormone after Ca2+ depletion of isolated frog skin epithelium.离体蛙皮上皮细胞Ca2+耗竭后,抗利尿激素对钠转运和环磷酸腺苷刺激的敏感性增强。
J Membr Biol. 1982;69(2):137-43. doi: 10.1007/BF01872273.
6
Sodium transport across the isolated epithelium of the frog skin.钠通过蛙皮分离上皮的转运。
J Physiol. 1971 Jan;212(1):195-210. doi: 10.1113/jphysiol.1971.sp009317.