Gootenberg J E, Ruscetti F W, Gallo R C
J Immunol. 1982 Oct;129(4):1499-505.
Three cell lines of mature T cell origin established from patients with cutaneous T cell lymphoma-leukemia (CTCL) have been found to be constitutive producers of TCGF (L-TCGF). Biologically active L-TCGF can also be eluted from the plasma membranes of these cells. We have compared the biologic and biochemical properties of L-TCGF and TCGF derived from normal lymphocytes (N-TCGF). L-TCGF and N-TCGF share similar biologic activity: both support long-term growth of T cells that have undergone prior lectin or antigen stimulation, and have no effect on unstimulated T cells. However, L-TCGF is a more acidic (pI 4.5 vs 6.5 to 8.0) molecule than N-TCGF and elutes from DEAE-Sepharose at higher salt concentration (0.2 M NaCl vs 0.07 to 0.1 M NaCl). In addition, these two factors display differing mobilities on gel filtration. Treatment of L-TCGF with neuraminidase or alkaline phosphatase does not alter its pI, indicating that enzymatically vulnerable sialic acid or phosphate groups are not involved in the variation. The nature and significance of this biochemical variant remain unknown.
已发现从皮肤T细胞淋巴瘤 - 白血病（CTCL）患者中建立的三种成熟T细胞来源的细胞系是TCGF（L - TCGF）的组成型生产者。具有生物活性的L - TCGF也可从这些细胞的质膜上洗脱下来。我们比较了L - TCGF和源自正常淋巴细胞的TCGF（N - TCGF）的生物学和生化特性。L - TCGF和N - TCGF具有相似的生物活性：两者都支持先前经过凝集素或抗原刺激的T细胞的长期生长，而对未刺激的T细胞没有影响。然而，L - TCGF是一种比N - TCGF更酸性的分子（pI 4.5对6.5至8.0），并在更高的盐浓度（0.2 M NaCl对0.07至0.1 M NaCl）下从DEAE - 琼脂糖中洗脱。此外，这两种因子在凝胶过滤中显示出不同的迁移率。用神经氨酸酶或碱性磷酸酶处理L - TCGF不会改变其pI，表明酶促敏感的唾液酸或磷酸基团不参与这种变化。这种生化变体的性质和意义仍然未知。
